Griffiths B B
Department of Microbiology/Immunology, School of Medicine, Oral Roberts University, Tulsa, Oklahoma 74137.
Microbios. 1989;58(236-237):135-46.
Seven strains of group B streptococci aetiologically related to humans were investigated in order to observe the role of glucose and medium iron on their growth and haemolysin production, and the necessity of a 'carrier' molecule to ensure the stability of the haemolysin. Both growth and haemolysin production were significantly influenced by glucose and medium iron concentrations. Iron concentrations between 5 and 7 micrograms/ml and 0.5% to 1% glucose were critical. Maximum haemolysin production (detected by lysis of sheep erythrocytes) was obtained during the late exponential phase of the growth cycle, but declined, producing sigmoidal-like curves as the organism entered the stationary phase. RNA-core, serum and bovine serum albumin, stabilizers of streptolysin S had no stabilizing effect for the haemolysin of group B streptococci. However, bovine serum albumin-starch mixture (0.5% bovine serum albumin and 0.5% starch in phosphate buffered saline or brain-heart infusion) was an effective stabilizer.
为了观察葡萄糖和培养基中铁对B族链球菌生长及溶血素产生的作用，以及“载体”分子对确保溶血素稳定性的必要性，对7株与人类病因相关的B族链球菌进行了研究。葡萄糖和培养基中铁的浓度对生长和溶血素产生均有显著影响。铁浓度在5至7微克/毫升之间以及0.5%至1%的葡萄糖是关键的。在生长周期的指数后期获得最大溶血素产量（通过绵羊红细胞裂解检测），但随着生物体进入稳定期，产量下降，产生类似S形的曲线。链球菌溶血素S的稳定剂RNA-核心、血清和牛血清白蛋白对B族链球菌的溶血素没有稳定作用。然而，牛血清白蛋白-淀粉混合物（在磷酸盐缓冲盐水或脑心浸液中含0.5%牛血清白蛋白和0.5%淀粉）是一种有效的稳定剂。
