三相流化床反应器中固定化反硝化副球菌对异丙醇的生物降解作用

Isopropanol biodegradation by immobilized Paracoccus denitrificans in a three-phase fluidized bed reactor.

作者信息

Geng Yucong, Deng Yuanjie, Chen Feilong, Jin Hong, Hou Taiping, Tao Ke

机构信息

a Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education , College of Life Sciences, Sichuan University , Chengdu , China.

出版信息

Prep Biochem Biotechnol. 2016 Nov 16;46(8):747-754. doi: 10.1080/10826068.2015.1135446.

DOI:10.1080/10826068.2015.1135446

PMID:26716611

Abstract

A three-phase bed bioreactor including a mix of immobilized microbes was used to degrade isopropanol (IPA). The immobilization method was studied and cells immobilized with calcium alginate, polyvinyl alcohol, activated carbon, and SiO were demonstrated to be the best immobilization method for the degradation of 90% of 2 g/L IPA in just 4 days, 1 day earlier than with free cells. Acetone was monitored as an indicator of microbial IPA utilization as the major intermediate of aerobic IPA biodegradation. The bioreactor was operated at hydraulic retention time (HRT) values of 32, 24, 16, 12, and 10 hr, which correspond to membrane fluxes of 0.03, 0.04, 0.06, 0.08, and 0.10 L/m/hr, respectively. The chemical oxygen demand (COD) removal efficiencies were maintained at 98.0, 97.8, 89.1, 80.6, and 71.1% at a HRT of 32, 24, 16, 12, and 10 hr, respectively, while the IPA degradations were 98.6, 98.3, 90.3, 81.6, and 73.3%, respectively. With a comprehensive consideration of COD removal and economy, the optimal HRT was 24 hr. The results demonstrate the potential of immobilized mixed bacterial consortium in a three-phase fluidized bed reactor system for the aerobic treatment of wastewater containing IPA.

摘要

使用包含固定化微生物混合物的三相床生物反应器来降解异丙醇（IPA）。研究了固定化方法，结果表明，用海藻酸钙、聚乙烯醇、活性炭和SiO固定的细胞是在短短4天内降解90%的2 g/L IPA的最佳固定化方法，比游离细胞提前1天。作为好氧IPA生物降解的主要中间体，监测丙酮作为微生物利用IPA的指标。生物反应器在水力停留时间（HRT）值为32、24、16、12和10小时下运行，分别对应于0.03、0.04、0.06、0.08和0.10 L/m/hr的膜通量。在HRT为32、24、16、12和10小时时，化学需氧量（COD）去除效率分别保持在98.0%、97.8%、89.1%、80.6%和7l.1%，而IPA降解率分别为98.6%、98.3%、90.3%、81.6%和73.3%。综合考虑COD去除和经济性，最佳HRT为24小时。结果证明了三相流化床反应器系统中固定化混合细菌群落对含IPA废水进行好氧处理的潜力。