酶联免疫吸附测定（ELISA）与使用32P或2-乙酰氨基芴（AAF）标记的cDNA探针进行斑点杂交用于检测和鉴定甜菜坏死黄脉病毒的比较。
Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus.
作者信息
Sakamoto H, Lemaire O, Merdinoglu D, Guesdon J L
机构信息
Laboratoire des Sondes Froides, Institut Pasteur, Paris, France.
出版信息
Mol Cell Probes. 1989 Jun;3(2):159-66. doi: 10.1016/0890-8508(89)90026-1.
Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybridization with non-radioactive probes and hybridization with radiolabelled probes were 2 ng, 0.2 ng, 0.02 ng of purified virus, respectively. Dot hybridization with non-radioactive probes could be performed on crude infected beet root extracts, thus providing a useful tool for monitoring BNYVV infection and for routine testing in plant breeding programs.
通过酶联免疫吸附测定（ELISA）以及使用放射性标记或非放射性探针的RNA/DNA斑点杂交来检测甜菜坏死黄脉病毒（BNYVV）。斑点杂交能够特异性地区分ELISA无法区分的分离株。ELISA、与非放射性探针杂交以及与放射性标记探针杂交的检测阈值分别为2纳克、0.2纳克、0.02纳克纯化病毒。与非放射性探针的斑点杂交可在感染的甜菜根粗提物上进行，从而为监测BNYVV感染以及植物育种计划中的常规检测提供了一种有用的工具。
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