Martin R R, D'Arcy C J
Agriculture Canada Research Station, Vancouver, B.C., Canada.
Intervirology. 1990;31(1):23-30. doi: 10.1159/000150130.
The luteoviruses barley yellow dwarf virus (BYDV-PAV and BYDV-RPV), bean leaf roll virus (BLRV) beet western yellows virus (BWYV), carrot red leaf virus, potato leaf roll virus, and soybean dwarf virus (SDV) were compared by hybridization with random cDNA probes and serologically with polyclonal antisera. For hybridizations, filters had each RNA blotted in duplicate dots at 10 ng/dot. Random-primed cDNA probes were prepared from 300 ng of each RNA and used to probe filters at three levels of stringency. Homologies were observed between BLRV and SDV and between BWYV and BYDV-RPV at the lowest level of stringency (Tm-38). In double-antibody sandwich enzyme-linked immunosorbent assay using polyclonal antisera, two-way relationships were observed between BWYV and BYDV-RPV. In indirect enzyme-linked immunosorbent assay, where purified virus denatured in pH 9.6 carbonate buffer was coated directly onto microtiter plates, relationships between members of the luteoviruses were much more extensive. BLRV, BWYV, BYDV-PAV, carrot red leaf virus, potato leaf roll virus, and SDV antisera reacted with each of the six luteoviruses tested in the indirect test, while the BYDV-RPV antiserum reacted only with BYDV-RPV, BWYV, and BLRV. BWYV and BYDV-RPV are closely related in both tests and should be considered strains of one virus.
通过与随机cDNA探针杂交以及使用多克隆抗血清进行血清学检测，对黄症病毒组的大麦黄矮病毒（BYDV - PAV和BYDV - RPV）、菜豆卷叶病毒（BLRV）、甜菜西方黄化病毒（BWYV）、胡萝卜红叶病毒、马铃薯卷叶病毒和大豆矮缩病毒（SDV）进行了比较。对于杂交实验，滤膜上每个RNA以10 ng/点的量重复点样两次。从每种RNA的300 ng制备随机引物cDNA探针，并用于在三种严谨度水平下检测滤膜。在最低严谨度水平（Tm - 38）下，观察到BLRV与SDV之间以及BWYV与BYDV - RPV之间存在同源性。在使用多克隆抗血清的双抗体夹心酶联免疫吸附测定中，观察到BWYV与BYDV - RPV之间存在双向关系。在间接酶联免疫吸附测定中，将在pH 9.6碳酸盐缓冲液中变性的纯化病毒直接包被在微量滴定板上，黄症病毒组成员之间的关系更为广泛。BLRV、BWYV、BYDV - PAV、胡萝卜红叶病毒、马铃薯卷叶病毒和SDV抗血清在间接检测中与所测试的六种黄症病毒中的每一种都发生反应，而BYDV - RPV抗血清仅与BYDV - RPV、BWYV和BLRV发生反应。在两种检测中，BWYV和BYDV - RPV都密切相关，应被视为一种病毒的株系。
