Aznar-Cervantes Salvador, Martínez Jose G, Bernabeu-Esclapez Antonia, Lozano-Pérez A Abel, Meseguer-Olmo Luis, Otero Toribio F, Cenis Jose L
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Department of Biotechnology La Alberca, Murcia E-30150, Spain.
Group for Electrochemistry, Intelligent Materials and Devices (GEMDI), Universidad Politécnica de Cartagena, ETSII, Cartagena, Murcia E-30203, Spain.
Bioelectrochemistry. 2016 Apr;108:36-45. doi: 10.1016/j.bioelechem.2015.12.003. Epub 2015 Dec 15.
Silk fibroin and graphene are both promising biomaterials described in the bibliography. Hybrid scaffolds combining their properties could be attractive for tissue engineering applications. In this work, a new methodology to produce electrospun fibroin scaffolds coated with graphene materials is provided. The mechanical, electrical and electrochemical properties of the materials attained were characterised. The fibre diameters were measured (from 3.9 to 5.2 μm). The samples coated with reduced grapheme were electronic conductors and electroactive in liquid electrolytes, showing maximum oxidation and reduction (around−0.4 V peak). The chronoamperometric responses showed a reduction shoulder, pointing to the entrance of balancing cations from the solution by nucleation–relaxation: the reaction induced structural changes in the graphene. In order to check the biocompatibility of the materials, they were seeded with L929 fibroblasts. The excellent biocompatibility of silk fibroin meshes was maintained after coating with graphene, being the proliferation results equal in all the treatments 7 days after the seeding (Tukey, p N 0.05).The conductive and electroactive properties of meshes coated with reduced graphene allow the potential application of local electric fields or local ionic currents to cell cultures, biological interfaces or animal models without host response.
丝素蛋白和石墨烯都是参考文献中所述的有前景的生物材料。结合它们特性的混合支架可能对组织工程应用具有吸引力。在这项工作中，提供了一种制备涂覆有石墨烯材料的电纺丝素蛋白支架的新方法。对所获得材料的机械、电学和电化学性质进行了表征。测量了纤维直径（3.9至5.2μm）。涂覆有还原石墨烯的样品是电子导体，并且在液体电解质中具有电活性，显示出最大氧化和还原（约 -0.4V峰值）。计时电流响应显示出一个还原峰，表明通过成核 - 弛豫从溶液中进入平衡阳离子：该反应引起石墨烯结构变化。为了检查材料的生物相容性，将L929成纤维细胞接种到材料上。用石墨烯涂覆后，丝素蛋白网的优异生物相容性得以保持，接种7天后所有处理的增殖结果相同（Tukey检验，p N 0.05）。涂覆有还原石墨烯的网的导电和电活性特性允许在没有宿主反应的情况下将局部电场或局部离子电流潜在地应用于细胞培养、生物界面或动物模型。
