Harvey R D, McHardy K C, Trainer P J, Reid I W
Neth J Med. 1989 Jun;34(5-6):302-5.
Thyrotrophin measured by the Amerwell immunoradiometric assay was found to be falsely elevated, causing potential diagnostic confusion in hypothyroid, euthyroid and hyperthyroid individuals. Non-specific antibodies in patients' sera may be responsible, by cross-linking the mouse monoclonal antibodies of the assay. Interference was overcome in the sera from these patients by addition of mouse serum (20 microliters per ml test serum) over and above that already incorporated in the kit. Problems with spuriously elevated thyrotrophin levels are not confined to one manufacturer's kit and can cause diagnostic confusion in several situations.
通过Amerwell免疫放射分析测定的促甲状腺激素被发现有假性升高，这在甲状腺功能减退、甲状腺功能正常和甲状腺功能亢进的个体中可能导致潜在的诊断混淆。患者血清中的非特异性抗体可能是造成这种情况的原因，它们会与该分析方法中的小鼠单克隆抗体发生交联。通过在试剂盒中已有的基础上额外添加小鼠血清（每毫升测试血清添加20微升），克服了这些患者血清中的干扰。促甲状腺激素水平假性升高的问题并不局限于某一制造商的试剂盒，并且在多种情况下都可能导致诊断混淆。
