Liu Xiao-peng, Wang Jan-fang, Qian Fei-yue, Wang Yan, Chen Chong-jun, Shen Yao-liang
Huan Jing Ke Xue. 2015 Sep;36(9):3352-7.
In order to evaluate the effect of organic loading rate ( OLR) on the formation of aerobic granular sludge (AGS), a lab-scale cylindrical SBR reactor (sodium acetate as carbon source) was constructed and inoculated with collected sewage sludge. The evolution of morphology, microbial activity and extracellular polymeric substances (EPS) characteristics of sludge samples in the reactor were recorded and analyzed. The results showed that AGS has the highest growth rate under the condition of 3. 20-4. 84 kg.(m3.d)-1 OLR, and a selective discharging strategy of the floccular sludge was suggested to maintain the predominance of AGS in reactor. The accumulated sludge concentration, SVI30, mean granule size, settling velocity and SOUR value of the AGS in steady-state operated SBR was 23. 9 g.L-1, 20 mL.g-1, 1. 4 mm, 102 m.h-1 and 50. 2 mg.(g.h)-1, respectively. The granulation process not only obviously changed the sludge appearance, but also significantly improved the microbial activity. Meanwhile, linear correlation was observed between the variation of protein/polysaccharide concentration and the granule size of AGS. Thus, variation of protein/ polysaccharide concentration of the EPS could be applied as an indicator for optimization of the cultivation method of AGS.
为了评估有机负荷率(OLR)对好氧颗粒污泥(AGS)形成的影响,构建了一个实验室规模的圆柱形SBR反应器(以乙酸钠为碳源),并用收集的污水污泥进行接种。记录并分析了反应器中污泥样品的形态、微生物活性和胞外聚合物(EPS)特性的演变。结果表明,在OLR为3.20 - 4.84 kg·(m³·d)⁻¹的条件下,AGS的生长速率最高,并提出了一种选择性排放絮状污泥的策略,以维持反应器中AGS的优势地位。稳态运行的SBR中AGS的累积污泥浓度、SVI₃₀、平均颗粒尺寸、沉降速度和SOUR值分别为23.9 g·L⁻¹、20 mL·g⁻¹、1.4 mm、102 m·h⁻¹和50.2 mg·(g·h)⁻¹。颗粒化过程不仅明显改变了污泥外观,还显著提高了微生物活性。同时,观察到蛋白质/多糖浓度的变化与AGS颗粒尺寸之间存在线性相关性。因此,EPS中蛋白质/多糖浓度的变化可作为优化AGS培养方法的指标。