用于高选择性富集磷酸肽的固定化Hf(4+)的聚多巴胺包覆磁性石墨烯的制备

Development of Hf(4+)-immobilized polydopamine-coated magnetic graphene for highly selective enrichment of phosphopeptides.

作者信息

Lin Haizhu, Deng Chunhui

机构信息

Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200433, China.

出版信息

Talanta. 2016;149:91-97. doi: 10.1016/j.talanta.2015.11.037. Epub 2015 Nov 18.

DOI:10.1016/j.talanta.2015.11.037

PMID:26717818

Abstract

In this work, we first designed and synthesized an IMAC material with Hf(4+) immobilized on polydopamine-coated magnetic graphene and applied it to phosphopeptides enrichment successfully. The newly prepared material gathered the advantages of large specific surface area of graphene, good hydrophilicity and biocompatibility of polydopamine and superparamagnetism of Fe3O4. We investigated the performance of Hf(4+)-immobilized polydopamine-coated magnetic graphene (denoted as magG@PDA-Hf(4+)) in phosphopeptides enrichment and the results showed high selectivity and sensitivity of the new material. Finally, we successfully applied magG@PDA-Hf(4+) to phosphopeptides enrichment from non-fat milk digests and human serum, further demonstrating excellent performance of this new material in phosphopeptides enrichment.

摘要

在本工作中，我们首先设计并合成了一种固定有Hf(4+)的IMAC材料，该材料负载于聚多巴胺包覆的磁性石墨烯上，并成功将其应用于磷酸化肽段的富集。新制备的材料兼具石墨烯大比表面积、聚多巴胺良好的亲水性和生物相容性以及Fe3O4的超顺磁性等优点。我们研究了固定有Hf(4+)的聚多巴胺包覆磁性石墨烯（记为magG@PDA-Hf(4+)）在磷酸化肽段富集中的性能，结果表明该新材料具有高选择性和高灵敏度。最后，我们成功将magG@PDA-Hf(4+)应用于从脱脂牛奶消化液和人血清中富集磷酸化肽段，进一步证明了这种新材料在磷酸化肽段富集中的优异性能。