C 反应蛋白分析测定法的生物分析进展。
Bioanalytical advances in assays for C-reactive protein.
机构信息
Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar der Technische Universität München, Ismaninger Str. 22, D-81675 Munich, Germany.
Department of Electrical and Computer Engineering, Jacobs School of Engineering, University of California San Diego, San Diego, CA 92093, U.S.A.
出版信息
Biotechnol Adv. 2016 May-Jun;34(3):272-90. doi: 10.1016/j.biotechadv.2015.12.010. Epub 2015 Dec 21.
This review presents advances in assays for human C-reactive protein (CRP), the most important biomarker of infection and inflammation for a plethora of diseases and pathophysiological conditions. Routine assays in clinical settings are based on analyzers, enzyme-linked immunosorbent assays and lateral flow assays. However, assays encompassing novel sensing schemes, improved chemistry, signal enhancement, lab-on-a-chip, microfluidics and smartphone detection, have emerged in recent years. The incorporation of immune-transducing chips or sensing interfaces with nanomaterials enables multiplexing analysis of CRP with co-existing biomarkers. However, there are still considerable challenges in the development of rapid diagnostics for both pentameric and monomeric CRP forms.
本综述介绍了用于检测人 C 反应蛋白（CRP）的分析方法的最新进展，CRP 是多种疾病和病理生理状况下感染和炎症的最重要生物标志物。临床常规检测方法基于分析仪、酶联免疫吸附测定法和侧向流动测定法。然而，近年来出现了一些新的检测方法，包括新型传感方案、改进的化学物质、信号增强、芯片实验室、微流控和智能手机检测等。将免疫转导芯片或具有纳米材料的传感界面与纳米材料结合使用，可以实现 CRP 与共存生物标志物的多重分析。然而，对于五聚体和单体 CRP 形式的快速诊断试剂的开发仍然存在很大的挑战。
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