Zhou Tianxiao, Leng Jiapeng, Peng Yaoshan, Zhang Lei, Guo Yinlong
Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai, PR China.
Department of Chemical Engineering, North University of China, Taiyuan, China.
J Sep Sci. 2016 Mar;39(5):873-9. doi: 10.1002/jssc.201501032. Epub 2016 Jan 25.
In combination with frozen pretreatment and carboxyl group derivatization, a novel workflow was developed for the determination of free fatty acids in milk powder. The workflow showed a significantly enhanced performance for comprehensive free fatty acid analysis owing to a highly efficient frozen extraction method. In addition, the advantages of the workflow also involved high sensitivity and great tolerance to a complex matrix. Characteristic fragment ions of derivatization reagents also provide clear evidence for the qualitative analysis of free fatty acids. Fourteen types of free fatty acids in a number of domestic and overseas infant milk powders have been successfully detected. The content of free fatty acids in the different samples was different, which probably indicates the diverse quality of infant milk powder. The workflow is expected to be a pragmatic tool for the analysis of free fatty acids in intricate matrices.
结合冷冻预处理和羧基衍生化，开发了一种用于测定奶粉中游离脂肪酸的新型工作流程。由于采用了高效的冷冻萃取方法，该工作流程在综合游离脂肪酸分析方面表现出显著增强的性能。此外，该工作流程的优点还包括高灵敏度和对复杂基质的高耐受性。衍生化试剂的特征碎片离子也为游离脂肪酸的定性分析提供了明确的证据。已成功检测出多种国内外婴儿奶粉中的14种游离脂肪酸。不同样品中游离脂肪酸的含量不同，这可能表明婴儿奶粉的质量存在差异。该工作流程有望成为分析复杂基质中游离脂肪酸的实用工具。
