Urata Shuzo, Weyer Jacqueline, Storm Nadia, Miyazaki Yukiko, van Vuren Petrus Jansen, Paweska Janusz Tadeusz, Yasuda Jiro
Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa.
J Virol. 2015 Dec 30;90(6):3257-61. doi: 10.1128/JVI.03198-15.
The recently identified arenavirus Lujo virus (LUJV) causes fatal hemorrhagic fever in humans. We analyzed its mechanism of viral release driven by matrix protein Z and the cell surface glycoprotein precursor GPC. The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding. LUJV GPC is cleaved by site 1 protease (S1P) at the RKLM motif, and treatment with the S1P inhibitor PF-429242 reduced LUJV production.
最近发现的沙粒病毒卢乔病毒（LUJV）可导致人类致命性出血热。我们分析了由基质蛋白Z和细胞表面糖蛋白前体GPC驱动的病毒释放机制。Z中的L结构域是有效释放病毒样颗粒所必需的，但Tsg101、ALIX/AIP1和Vps4A/B对出芽并非必需。LUJV GPC在RKLM基序处被1型位点蛋白酶（S1P）切割，用S1P抑制剂PF-429242处理可降低LUJV的产生。
