人类内源性逆转录病毒K转导所需序列的确定及其被外源逆转录病毒粒子识别的研究
Determination of Sequences Required for Human Endogenous Retrovirus K Transduction and Its Recognition by Foreign Retroviral Virions.
作者信息
Erlwein Otto, Sweeney Nathan P, de Leon Raffaele, Wills Gillian, Robinson Mark J, McClure Myra O
机构信息
Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom.
Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Wright-Fleming Institute, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London, United Kingdom
出版信息
J Virol. 2015 Dec 30;90(6):3243-6. doi: 10.1128/JVI.02731-15.
Sequences necessary for transduction of human endogenous retrovirus (HERV)-Kcon, a consensus of the HERV-K(HML-2) family, were analyzed and found to reside in the leader/gag region. They act in an orientation-dependent way and consist of at least two sites working together. Having defined these sequences, we exploited this information to produce a simple system to investigate to what extent virions of HERV-Kcon, murine leukemia virus, and HIV-1 have the ability to transduce each other's genomes, leading to potential contamination of gene therapy vectors.
对人类内源性逆转录病毒（HERV）-Kcon（HERV-K（HML-2）家族的一个共有序列）转导所需的序列进行了分析，发现其位于前导/ gag区域。它们以方向依赖的方式起作用，并且由至少两个共同起作用的位点组成。确定这些序列后，我们利用这些信息构建了一个简单的系统，以研究HERV-Kcon、鼠白血病病毒和HIV-1的病毒粒子在多大程度上能够相互转导彼此的基因组，从而导致基因治疗载体的潜在污染。
Zotero 插件