State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Hengte Heavy Industry Co. Ltd., Dezhou, China.
Bioresour Technol. 2016 Mar;203:1-10. doi: 10.1016/j.biortech.2015.12.040. Epub 2015 Dec 18.
The dynamic changes in the composition and function of both bacterial and fungal communities over time and at various depths in the compost of a 90-m(3) industrial-scale fermentor were explored using integrated meta-omics. The microbial communities in the middle layer (1.2m) of the compost developed a stable and simple structure over time, which was mainly composed of Thermobifida, Bacillus, Thermomyces and Aspergillus. According to the metaproteomic results, the bacterial community was more focused on cellulose degradation, characterized by 44% of the cellulases that were secreted by Thermobifida, while the fungal community was more likely to degrade hemicellulose, mainly via Thermomyces and Aspergillus. The results revealed that, under artificial control of the temperature and oxygen concentration, the efficiency of organic waste degradation was greatly increased and the fermentation cycle was shortened to 11 days.
采用集成宏基因组学方法，研究了 90 立方米工业规模发酵罐堆肥中细菌和真菌群落组成和功能随时间和不同深度的动态变化。堆肥中层（1.2m）的微生物群落随时间推移形成了稳定而简单的结构，主要由 Thermobifida、芽孢杆菌、嗜热丝孢菌和曲霉组成。根据宏蛋白质组学结果，细菌群落更专注于纤维素降解，Thermobifida 分泌的 44%的纤维素酶就是特征之一，而真菌群落更有可能降解半纤维素，主要通过嗜热丝孢菌和曲霉。结果表明，在温度和氧气浓度的人工控制下，有机废物降解效率大大提高，发酵周期缩短至 11 天。
