Liu Xia, Li Dong, Liu Qiang, Yang Bin, Wang Xiaojing, Zhang Yan, Niu Yan, Yang Chunxia, Zhang Yu
Institute of quality standards and testing technology for Agro products, Ningxia academy of agriculture and forestry sciences, Yinchuan, 750002, China.
Center for transformation of scientific and technological achievements, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.
Heliyon. 2024 Nov 3;10(22):e40013. doi: 10.1016/j.heliyon.2024.e40013. eCollection 2024 Nov 30.
The incorporation of honeysuckle as a silage additive in alfalfa production has yielded promising results; however, the underlying microbial mechanisms during fermentation remain poorly understood. This study leveraged high-throughput sequencing and nutrient profiling to elucidate microbial population dynamics over 45 days of anaerobic fermentation within a vacuum-assisted co-culture system comprising varying fresh weight ratios of honeysuckle branches, , and alfalfa. The experimental design encompassed a -supplemented treatment (M group) and an unsupplemented control (R group), each with five honeysuckle percentages (5 %, 10 %, 15 %, 20 %, 25 %) and respective negative controls. Our findings reveal that the combined use of honeysuckle branches and significantly impacts microbial community composition and diversity during alfalfa silage fermentation. The Mantel test underscores 's absence-dependent correlation of bacterial communities with pH and lactic acid, whereas its presence redirects these correlations towards total nitrogen, NH -N, neutral detergent fiber, and crude protein as key bacterial population drivers. Fungal populations exhibited analogous trends. Moreover, the combined additives reshaped microbial populations during anaerobic fermentation, altering interaction networks and intensifying microbial interplay. Notably, introduction on a honeysuckle branch base diminished fungal core OTUs, thereby mitigating fungal contamination risks. This study also identified biomarkers unique to each treatment condition. Collectively, our research provides a molecular framework for employing honeysuckle branches and as innovative silage additives in alfalfa production, fostering the development of eco-friendly and hygienic feed and farming industries.
在苜蓿生产中使用金银花作为青贮添加剂已取得了令人鼓舞的成果；然而，发酵过程中潜在的微生物机制仍知之甚少。本研究利用高通量测序和营养成分分析，以阐明在一个包含不同鲜重比的金银花枝条、[此处原文缺失部分内容]和苜蓿的真空辅助共培养系统中，厌氧发酵45天内微生物种群的动态变化。实验设计包括一个添加[此处原文缺失部分内容]的处理组（M组）和一个未添加的对照组（R组），每组有五个金银花百分比（5%、10%、15%、20%、25%）及各自的阴性对照。我们的研究结果表明，金银花枝条和[此处原文缺失部分内容]的联合使用对苜蓿青贮发酵过程中的微生物群落组成和多样性有显著影响。Mantel检验强调，[此处原文缺失部分内容]不存在时细菌群落与pH值和乳酸呈相关性，而其存在时则将这些相关性转向总氮、NH₃-N、中性洗涤纤维和粗蛋白，将其作为关键细菌种群驱动因素。真菌种群呈现出类似趋势。此外，联合添加剂在厌氧发酵过程中重塑了微生物种群，改变了相互作用网络并增强了微生物间的相互作用。值得注意的是，在金银花枝条基础上添加[此处原文缺失部分内容]减少了真菌核心OTU，从而降低了真菌污染风险。本研究还确定了每种处理条件下独特的生物标志物。总体而言，我们的研究为在苜蓿生产中使用金银花枝条和[此处原文缺失部分内容]作为创新型青贮添加剂提供了一个分子框架，促进了生态友好型和卫生型饲料及养殖行业的发展。
