智利养蜂场中近期发现的巴氏洛特鞭毛虫（锥虫科）的PCR特异性检测
PCR-specific detection of recently described Lotmaria passim (Trypanosomatidae) in Chilean apiaries.
作者信息
Arismendi Nolberto, Bruna Alex, Zapata Nelson, Vargas Marisol
机构信息
Laboratories of Virology and Bee Pathology, Faculty of Agronomy, Universidad de Concepción, Av. Vicente Méndez 595, Chillán, Chile.
Departament of Plant Production, Faculty of Agronomy, Universidad de Concepción, Av. Vicente Méndez 595, Chillán, Chile.
出版信息
J Invertebr Pathol. 2016 Feb;134:1-5. doi: 10.1016/j.jip.2015.12.008. Epub 2015 Dec 22.
The recently described trypanosome Lotmaria passim is currently considered the most predominant trypanosomatid in honey bees worldwide and could be a factor in honey bee declines. For a specific and quick detection of this pathogen, we developed primers based on the SSU rRNA and gGAPDH genes for the detection of L. passim in Chilean honey beehives. PCR products amplified and sequenced for these primers shared 99-100% identity with other sequences of L. passim. The designed primers were specific and we were able to detect a high prevalence (40-90%) of L. passim in bee hives distributed throughout Chile. Our described PCR-based method offers a feasible and specific detection of L. passim in any honey bee samples.
最近描述的锥虫Lotmaria passim目前被认为是全球蜜蜂中最主要的锥虫，可能是蜜蜂数量减少的一个因素。为了特异性快速检测这种病原体，我们基于小亚基核糖体RNA（SSU rRNA）和甘油醛-3-磷酸脱氢酶（gGAPDH）基因设计了引物，用于检测智利蜂巢中的L. passim。这些引物扩增并测序得到的PCR产物与L. passim的其他序列具有99%-100%的同一性。设计的引物具有特异性，我们能够在智利各地分布的蜂巢中检测到高流行率（40%-90%）的L. passim。我们描述的基于PCR的方法为在任何蜜蜂样本中检测L. passim提供了一种可行且特异的检测方法。
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