Yu Wilson, Walling Ian, Smith Autumn B, Ramirez-Zamora Adolfo, Pilitsis Julie G, Shin Damian S
Center for Neuropharmacology & Neuroscience, Albany Medical College, Albany, NY, USA.
Department of Neurology, Albany Medical Center, Albany, NY, USA.
Brain Stimul. 2016 Mar-Apr;9(2):285-95. doi: 10.1016/j.brs.2015.11.006. Epub 2015 Nov 26.
Brain stimulation is effective for people with intractable epilepsy. However, modulating neural targets that provide greater efficacy to more individuals is still needed.
OBJECTIVE/HYPOTHESIS: We investigate whether bilateral deep brain stimulation of the ventral pallidum (VP-DBS) has potent seizure control in pilocarpine-treated rats.
VP-DBS (50 Hz) was applied prior to generalized forebrain seizures or after generalized brainstem seizures manifested. Behavioral seizures were assessed using a modified Racine scale. In vitro and in vivo electrophysiological techniques were employed to identify how VP-DBS affects proximal and distal neuronal activity. The open field test was used to see if acute and chronic VP-DBS affected gross motor function or arousal state. Parametric and non-parametric statistics with post-hoc analysis were performed.
VP-DBS prior to pilocarpine prevented behavioral forebrain and brainstem seizures in most animals (n = 15). VP-DBS after brainstem seizures emerged prevented or reduced the appearance of subsequent behavioral brainstem seizures (n = 11). VP-DBS attenuated epileptiform activity in the hippocampus (n = 5), but not in the primary somatosensory cortex (S1) (n = 4) in vivo. Electrical stimulation in the VP increased VP GABAergic neuronal firing activity from 3.1 ± 1.4 Hz to 7.6 ± 1.7 Hz (n = 8) in vitro and reduced substantia nigra reticulata and superior colliculus neuronal spiking activity from 25.4 ± 3.3 Hz to 18.2 ± 1.4 Hz (n = 6) and 18.2 ± 1.4 Hz to 11.0 ± 1.1 Hz (n = 18), respectively, in vivo.
VP-DBS can be a novel and potent therapeutic approach for individuals with intractable epilepsy.
脑刺激对难治性癫痫患者有效。然而，仍需要调节能为更多个体带来更高疗效的神经靶点。
目的/假设：我们研究双侧苍白球腹侧深部脑刺激（VP-DBS）对毛果芸香碱处理的大鼠是否具有有效的癫痫控制作用。
在全身性前脑癫痫发作之前或全身性脑干癫痫发作出现之后施加VP-DBS（50赫兹）。使用改良的拉辛量表评估行为性癫痫发作。采用体外和体内电生理技术来确定VP-DBS如何影响近端和远端神经元活动。使用旷场试验来观察急性和慢性VP-DBS是否影响总体运动功能或觉醒状态。进行了参数和非参数统计以及事后分析。
在毛果芸香碱处理之前进行VP-DBS可预防大多数动物（n = 15）的行为性前脑和脑干癫痫发作。脑干癫痫发作出现后进行VP-DBS可预防或减少随后行为性脑干癫痫发作的出现（n = 11）。VP-DBS减弱了体内海马体中的癫痫样活动（n = 5），但在初级体感皮层（S1）中未减弱（n = 4）。体外实验中，VP内的电刺激使VP内γ-氨基丁酸能神经元的放电活动从3.1±1.4赫兹增加到7.6±1.7赫兹（n = 8），体内实验中，分别使黑质网状部和上丘神经元的放电活动从25.4±3.3赫兹降低到18.2±1.4赫兹（n = 6）以及从18.2±1.4赫兹降低到11.0±1.1赫兹（n = 18）。
VP-DBS可能是一种针对难治性癫痫患者的新型有效治疗方法。
