Kornienko Maria, Ilina Elena, Lubasovskaya Ludmila, Priputnevich Tatiana, Falova Oksana, Sukhikh Gennadiy, Govorun Vadim
Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russia.
Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russia.
Infect Genet Evol. 2016 Apr;39:99-105. doi: 10.1016/j.meegid.2015.12.015. Epub 2015 Dec 23.
Coagulase-negative staphylococci (CoNS) are a major component of normal human skin and mucosae flora. However, some species of CoNS can lead to infections in immunocompromised patients and premature newborns. The choice of a rapid and reliable typing method is one of the major problems in the epidemiological monitoring of CoNS, especially Staphylococcushaemolyticus isolates. In this study, we have tested 71 isolates of S. haemolyticus obtained from newborns using the multilocus sequence typing (MLST) and the direct bacterial MALDI-TOF mass spectrometry profiling approaches. To date, there is no standard MLST scheme for investigating the diversity of S. haemolyticus strains. The novel variant of MLST scheme including the tpiA, pta, sh1200, rphE, tphK, mvaK1, and arc loki was tested. The discriminatory power was estimated by the Hunter-Gaston discriminatory index (D) as 0.95. The Composition Correlation Index Matrix (CCI matrix) was calculated to typing the isolates through the analysis of mass spectra; also, the values of the correlation index for different groups of isolates were evaluated. Closely related isolates obtained from the same hospital are characterized by increased values of correlation indices in comparison with these values of isolates collected from various hospitals. The data obtained by both methods allow to describe a clonal structure of S. haemolyticus population and to designate the presence of endemic clones of S. haemolyticus.
凝固酶阴性葡萄球菌（CoNS）是人类正常皮肤和黏膜菌群的主要组成部分。然而，某些CoNS菌种可导致免疫功能低下患者和早产儿感染。选择一种快速可靠的分型方法是CoNS流行病学监测中的主要问题之一，尤其是溶血葡萄球菌分离株。在本研究中，我们使用多位点序列分型（MLST）和直接细菌基质辅助激光解吸电离飞行时间质谱分析方法，对从新生儿中获得的71株溶血葡萄球菌分离株进行了检测。迄今为止，尚无用于研究溶血葡萄球菌菌株多样性的标准MLST方案。我们测试了包括tpiA、pta、sh1200、rphE、tphK、mvaK1和arc loki在内的MLST方案新变体。通过Hunter-Gaston鉴别指数（D）估计鉴别力为0.95。计算组成相关指数矩阵（CCI矩阵）以通过质谱分析对分离株进行分型；此外，还评估了不同分离株组的相关指数值。与从不同医院收集的分离株相比，从同一医院获得的密切相关分离株的相关指数值更高。两种方法获得的数据都有助于描述溶血葡萄球菌群体的克隆结构，并确定溶血葡萄球菌地方流行克隆的存在。
