State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2016 Mar;203:220-7. doi: 10.1016/j.biortech.2015.12.032. Epub 2015 Dec 18.
In order to produce biodiesel from microalgae cultured with abundant seawater, Chlorella sp. was mutated with (137)Se-γ ray irradiation and domesticated with f/2 seawater culture medium (salinity=3 wt.%) under 15 vol.% CO2 stress. Biomass yield of the mutant increased by 25% compared with wild species and lipid content increased to 54.9%. When nitrogen and phosphorus concentrations in the initial substrate increased, the increased propagation speed of the mutant resulted in decreased cell diameter by 26.6% and decreased cell wall thickness by 69.7%. The dramatically increased biomass yield of the mutant with sufficient initial substrate and relative nitrogen starvation in the later growth period with continuous 15 vol.% CO2 led to an increased lipid yield of 1.0 g/L. The long-chain unsaturated fatty acids increased, whereas short-chain saturated fatty acids decreased.
为了利用富海水培养的微藻生产生物柴油，采用 (137)Se-γ 射线辐照对小球藻进行诱变，并在 15 vol.% CO2 胁迫下用 f/2 海水培养基（盐度=3 wt.%）进行驯化。与野生种相比，突变体的生物量增加了 25%，脂质含量增加到 54.9%。当初始基质中的氮磷浓度增加时，突变体的增殖速度加快，导致细胞直径减小 26.6%，细胞壁厚度减小 69.7%。在后期生长过程中，充足的初始基质和相对氮饥饿以及持续的 15 vol.% CO2 导致突变体的生物量产量显著增加，脂质产量增加到 1.0 g/L。长链不饱和脂肪酸增加，而短链饱和脂肪酸减少。
