Lee Dahae, Ko Hyeonseok, Kim Young-Joo, Kim Su-Nam, Choi Kyung-Chul, Yamabe Noriko, Kim Ki Hyun, Kang Ki Sung, Kim Hyun Young, Shibamoto Takayuki
College of Korean Medicine, Gachon University , Seongnam 13120, Korea.
Department of Food Science, Gyeongnam National University of Science and Technology , Jinju 660-758, Korea.
J Agric Food Chem. 2016 Feb 3;64(4):801-5. doi: 10.1021/acs.jafc.5b05461. Epub 2016 Jan 19.
The effects of a red raspberry component, sanguiin H-6 (SH-6), on the induction of apoptosis and the related signaling pathways in A2780 human ovarian carcinoma cells were investigated. SH-6 caused an antiproliferative effect and a severe morphological change resembling that of apoptotic cell death but no effect on the cancer cell cycle arrest. In addition, SH-6 induced an early apoptotic effect and activation of caspases as well as the cleavage of PARP, which is a hallmark of apoptosis. The early apoptotic percentages of A2780 cells exposed to 20 and 40 μM SH-6 were 35.39 and 41.76, respectively. Also, SH-6 caused the activation of mitogen-activated protein kinases (MAPKs), especially p38, and the increase of truncated p15/BID. These results in the present study suggest that the apoptosis of A2780 human ovarian carcinoma cells by SH-6 is mediated by the MAPK p38 and a caspase-8-dependent BID cleavage pathway.
研究了红树莓成分桑色素H-6（SH-6）对A2780人卵巢癌细胞凋亡诱导及相关信号通路的影响。SH-6具有抗增殖作用，并引起类似于凋亡细胞死亡的严重形态变化，但对癌细胞周期停滞无影响。此外，SH-6诱导早期凋亡效应、半胱天冬酶激活以及PARP裂解，这是凋亡的一个标志。暴露于20和40μM SH-6的A2780细胞的早期凋亡率分别为35.39和41.76。此外，SH-6导致丝裂原活化蛋白激酶（MAPK）尤其是p38的激活以及截短型p15/BID的增加。本研究的这些结果表明，SH-6诱导A2780人卵巢癌细胞凋亡是由MAPK p38和半胱天冬酶-8依赖性BID裂解途径介导的。
