Kim Seyeon, Park Chanoong, Gang Jongback
J Nanosci Nanotechnol. 2015 Oct;15(10):7913-7. doi: 10.1166/jnn.2015.11217.
Graphene oxide (GO) has a large surface-to-volume ratio and hydrophobic hexagonal rings that can interact with biomolecules. Single-stranded DNA adsorbs strongly to the surface of GO via hydrophobic interactions. GO has been used in optical biosensors and biomedical platforms for the detection of DNA, proteins, and small molecules. This study was designed to measure the adsorption of double-stranded DNA (dsDNA) onto GO according to DNA length, salt concentration, and pH of the reaction. Results showed that dsDNA molecules were adsorbed progressively as the pH changed from 6.0 to 4.0. At high pH, dsDNA adsorption was enhanced by the presence of MgCl2 rather than NaCl. Desorption of DNA from GO, with triton X-100 led to the rapid release of DNA from GO in the presence of MgCl2.
氧化石墨烯（GO）具有较大的表面积与体积比以及可与生物分子相互作用的疏水六元环。单链DNA通过疏水相互作用强烈吸附于GO表面。GO已被用于光学生物传感器和生物医学平台，用于检测DNA、蛋白质和小分子。本研究旨在根据DNA长度、盐浓度和反应pH值来测量双链DNA（dsDNA）在GO上的吸附情况。结果表明，随着pH值从6.0变为4.0，dsDNA分子逐渐被吸附。在高pH值下，MgCl2的存在而非NaCl能增强dsDNA的吸附。在MgCl2存在的情况下，用曲拉通X-100从GO上解吸DNA会导致DNA从GO上快速释放。
