Grabowski E F
Division of Pediatric Hematology/Oncology, New York Hospital-Cornell University Medical Center, New York 10021.
Am J Cardiol. 1989 Sep 5;64(9):10E-15E. doi: 10.1016/0002-9149(89)90728-5.
To compare the biocompatibility of nonionic and ionic intravascular contrast media in a more physiologic in vitro system, key aspects of blood flow through a microvessel were simulated. Toward this end, monolayers of endothelial cells placed in a specially designed flow chamber, real-time imaging of the monolayers by brightfield and phase videomicroscopy, and real-time imaging and computer-aided quantitation at normal hematocrit levels of platelet adhesion/aggregation to sites of monolayer injury by means of epifluorescence videomicroscopy were used. At a concentration in culture medium of 20% by volume, it was found that monolayer morphology was least altered by iohexol when compared with diatrizoate and ioxaglate; monolayer production of prostacyclin was enhanced (p less than 0.001) by ioxaglate compared with saline controls; and at a concentration in citrated blood of 20% by non-red-cell volume, platelet adhesion/aggregation was reduced by all 3 contrast agents in the order diatrizoate greater than ioxaglate greater than iohexol.
为了在更接近生理状态的体外系统中比较非离子型和离子型血管内造影剂的生物相容性，模拟了血液流经微血管的关键方面。为此，将内皮细胞单层置于专门设计的流动腔室中，通过明场和相衬视频显微镜对单层进行实时成像，并通过落射荧光视频显微镜在正常血细胞比容水平下对血小板黏附/聚集到单层损伤部位进行实时成像和计算机辅助定量分析。当培养基中体积浓度为20%时，发现与泛影葡胺和碘克沙醇相比，碘海醇对单层形态的改变最小；与生理盐水对照相比，碘克沙醇可增强单层前列环素的生成（p<0.001）；在枸橼酸盐血液中，当非红细胞体积浓度为20%时，所有3种造影剂均可降低血小板黏附/聚集，其顺序为泛影葡胺>碘克沙醇>碘海醇。
