Wei Hanxiao, Li Caixia, Zhao Xian, Yuan Ruihong, Dai Xiaoming, Li Yisong, Liu Liu
Department of Plastic Surgery, First Affiliated Hospital, Kunming Medical University, Kunming 650032, China.
Department of Plastic Surgery, First Affiliated Hospital, Kunming Medical University, Kunming 650032, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Jan;32(1):20-4.
To investigate the effects of peripheral blood endothelial progenitor cells (PB-EPCs) on the homing ability of bone marrow stromal cells (BMSCs) as well as the potential mechanism.
BMSCs were injected intravenously with lentiviral expression vector expressing enhanced green fluorescent protein (EGFP) for tracing. Biological bone graft was made to repair rabbit radial defect. In the experimental group, PB-EPCs and BMSCs mixed at a ratio of 1:1 were combined with partially deproteinized bone (PDPB) for implantation to repair rabbit models with radial bone defect. BMSCs alone were combined with PDPB in the control group. The models in the blank group were not repaired. Protein and mRNA levels of endogenous stromal-derived factor-1 (SDF-1) and monocyte chemotactic protein-1 (MCP-1) were evaluated by ELISA and real-time quantitative PCR at 2, 4, 8 weeks after the operation. At the same time points, immunohistochemical staining was performed to detect EGFP expression in the defect sites.
The mRNA and protein levels of SDF-1 and MCP-1 in the experimental group were higher than those in the other two groups. Immunohistochemistry showed that the number of EGFP-positive cells was larger in the experimental group than in the control or the blank group.
PB-EPCs can increase the expressions of SDF-1 and MCP-1 and promote the migration of EGFP-positive BMSCs to bone defect site.
探讨外周血内皮祖细胞（PB-EPCs）对骨髓基质细胞（BMSCs）归巢能力的影响及其潜在机制。
通过静脉注射携带增强型绿色荧光蛋白（EGFP）的慢病毒表达载体来标记BMSCs。制备生物骨移植物修复兔桡骨缺损。实验组将PB-EPCs与BMSCs按1:1比例混合后与部分脱蛋白骨（PDPB）联合植入，用于修复兔桡骨缺损模型。对照组仅将BMSCs与PDPB联合。空白组模型不进行修复。术后2、4、8周，采用酶联免疫吸附测定（ELISA）和实时定量PCR评估内源性基质细胞衍生因子-1（SDF-1）和单核细胞趋化蛋白-1（MCP-1）的蛋白和mRNA水平。在相同时间点，进行免疫组织化学染色检测缺损部位的EGFP表达。
实验组中SDF-1和MCP-1的mRNA及蛋白水平高于其他两组。免疫组织化学显示，实验组中EGFP阳性细胞数量多于对照组和空白组。
PB-EPCs可增加SDF-1和MCP-1的表达，并促进EGFP阳性的BMSCs向骨缺损部位迁移。
