Eanes E D
Bone Research Branch Research Associate Program, National Institute of Dental Research, National Bureau of Standards, Gaithersburg, Maryland 20899.
Anat Rec. 1989 Jun;224(2):220-5. doi: 10.1002/ar.1092240211.
The present paper reviews the use of liposomes as synthetic models for studying various biophysical aspects of matrix vesicle calcification, especially the involvement of acidic phospholipids in the nucleation and growth processes which occur during the initial stages of mineral formation in and around these membrane-bound structures. Recent results showed that acidic phospholipids incorporated into phosphatidylcholine-rich anionic liposome membranes were ineffective in initiating extraliposomal calcium phosphate precipitation from metastable solutions at physiological pH. On the contrary, certain acidic phospholipids such as phosphatidic acid and phosphatidylserine retarded the development of such precipitation when the latter was endogenously induced. The extent of inhibition correlated with the strength of the electrostatic interaction between the polar head group of the acidic phospholipid and the surface of the mineral phase. The results suggest that acidic phospholipids may play an important role in controlling the rate of early mineral development in matrix vesicle calcification.
本文综述了脂质体作为合成模型在研究基质小泡钙化的各种生物物理方面的应用，特别是酸性磷脂在这些膜结合结构内外矿物质形成初始阶段发生的成核和生长过程中的作用。最近的结果表明，掺入富含磷脂酰胆碱的阴离子脂质体膜中的酸性磷脂在生理pH值下无法从亚稳溶液引发脂质体外磷酸钙沉淀。相反，当内源性诱导后者时，某些酸性磷脂如磷脂酸和磷脂酰丝氨酸会抑制这种沉淀的发展。抑制程度与酸性磷脂的极性头部基团与矿相表面之间的静电相互作用强度相关。结果表明，酸性磷脂可能在控制基质小泡钙化早期矿物质发育速率方面发挥重要作用。
