Chen Jiong, Xu Hong, Zhu Xing-Xing
Department of General Surgery, Affiliated Provincial Hospital, Anhui Medical University, Hefei, People's Republic of China; Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Hefei, People's Republic of China.
Ther Clin Risk Manag. 2015 Dec 22;12:11-8. doi: 10.2147/TCRM.S96869. eCollection 2016.
Soluble major histocompatibility complex class I-related chain A molecules (sMICA) and natural-killer group 2 member D (NKG2D) not only correlate with tumorigenesis and progression, but also with tumor invasion and metastasis. In this study, we used immunohistochemistry to investigate the correlation and prognostic significance of the differential expression of sMICA and NKG2D in pancreatic carcinoma and paracarcinoma tissues from 70 patients with pancreatic carcinomas. The results showed that sMICA expression was significantly (P<0.05) higher in tumor tissues (67.1%) than that in adjacent nontumor tissues (31.4%), whereas NKG2D expression was significantly (P<0.001) lower in tumor tissues (32.9%) than that in adjacent nontumor tissues (60.0%). Spearman's rank correlation test showed a negative correlation between the expression of sMICA and that of NKG2D (r=-0.676, P<0.001). Kaplan-Meier survival analysis showed that a high sMICA expression was significantly correlated with decreased disease-free survival (DFS) (P<0.001) and overall survival (OS) (P<0.001), while a high NKG2D expression was significantly associated with increased DFS (P=0.001) and OS (P=0.001) of the patients. Multivariate analysis showed that a high sMICA expression was an independent predictive factor for poor DFS (P<0.001) and OS (P=0.012); but low NKG2D expression was not an independent prognostic factor for poor DFS (P=0.238) and OS (P=0.574). In conclusion, our findings suggest that the expression levels of sMICA and NKG2D are abnormal and negatively correlated with one another in pancreatic carcinoma tissues; they may be considered as valuable biomarkers for the prognosis of pancreatic carcinoma.
可溶性主要组织相容性复合体I类相关链A分子（sMICA）和自然杀伤细胞2族成员D（NKG2D）不仅与肿瘤发生和进展相关，还与肿瘤侵袭和转移相关。在本研究中，我们采用免疫组织化学方法，调查了70例胰腺癌患者的癌组织和癌旁组织中sMICA和NKG2D差异表达的相关性及预后意义。结果显示，肿瘤组织中sMICA的表达（67.1%）显著高于癌旁非肿瘤组织（31.4%）（P<0.05），而肿瘤组织中NKG2D的表达（32.9%）显著低于癌旁非肿瘤组织（60.0%）（P<0.001）。Spearman等级相关检验显示，sMICA与NKG2D的表达呈负相关（r=-0.676，P<0.001）。Kaplan-Meier生存分析显示，sMICA高表达与无病生存期（DFS）缩短（P<0.001）和总生存期（OS）缩短（P<0.001）显著相关，而NKG2D高表达与患者DFS延长（P=0.001）和OS延长（P=0.001）显著相关。多因素分析显示，sMICA高表达是DFS不良（P<0.001）和OS不良（P=0.012）的独立预测因素；但NKG2D低表达不是DFS不良（P=0.238）和OS不良（P=0.574）的独立预后因素。总之，我们的研究结果表明，胰腺癌组织中sMICA和NKG2D的表达水平异常且呈负相关；它们可被视为胰腺癌预后的有价值生物标志物。
