Jia Hong-Ying, Liu Jun-Li, Zhou Cheng-Jun, Kong Feng, Yuan Ming-Zhen, Sun Wen-Dong, Wang Jue, Liu Ling, Zhao Jing-Jie, Luan Yun
Department of Evidence-Based Medicine, The Second Hospital, Shandong University, Jinan, China E-mail :
Asian Pac J Cancer Prev. 2014;15(4):1715-7. doi: 10.7314/apjcp.2014.15.4.1715.
The overall incidence and mortality of renal cell carcinoma (RCC), the most common kidney cancer, are steadily increasing for reasons that are not fully explained. Our aim was to explore the expression of membrane MHC class I chain-related gene A (mMICA) in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA (sMICA) in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA) . The results showed that percentage of mMICA expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P<0.05). sMICA content in healthy adults was negative, but in renal cancer patients was significantly elevated (P<0.05). Our research showed that high expression of MICA in human kidney cancer, this results show that MICA might serve as potential tumor-associated antigen (TAA) in RCC.
最常见的肾癌——肾细胞癌（RCC）的总体发病率和死亡率正在稳步上升，原因尚未完全明确。我们的目的是探讨膜MHC I类链相关基因A（mMICA）在人肾癌细胞系和组织标本中的表达，并通过流式细胞术（FCM）、免疫组织化学以及酶联免疫吸附测定（ELISA）来测定肾细胞癌患者血清中可溶性MICA（sMICA）的表达。结果显示，与健康成年人及人胚肾293（HEK293）细胞系个体相比，人肾癌组织和肾癌细胞系（786 - O和Ketr - 3）中mMICA表达百分比显著升高（P<0.05）。健康成年人的sMICA含量为阴性，但在肾癌患者中显著升高（P<0.05）。我们的研究表明，MICA在人肾癌中高表达，这表明MICA可能作为肾细胞癌潜在的肿瘤相关抗原（TAA）。
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