Unamba Chibuikem I N, Nag Akshay, Sharma Ram K
Biotechnology Division, CSIR-Institute of Himalayan Bioresource TechnologyPalampur, India; Department of Plant Science and Biotechnology, Imo State UniversityOwerri, Nigeria.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology Palampur, India.
Front Plant Sci. 2015 Dec 16;6:1074. doi: 10.3389/fpls.2015.01074. eCollection 2015.
Non-model plants i.e., the species which have one or all of the characters such as long life cycle, difficulty to grow in the laboratory or poor fecundity, have been schemed out of sequencing projects earlier, due to high running cost of Sanger sequencing. Consequently, the information about their genomics and key biological processes are inadequate. However, the advent of fast and cost effective next generation sequencing (NGS) platforms in the recent past has enabled the unearthing of certain characteristic gene structures unique to these species. It has also aided in gaining insight about mechanisms underlying processes of gene expression and secondary metabolism as well as facilitated development of genomic resources for diversity characterization, evolutionary analysis and marker assisted breeding even without prior availability of genomic sequence information. In this review we explore how different Next Gen Sequencing platforms, as well as recent advances in NGS based high throughput genotyping technologies are rewarding efforts on de-novo whole genome/transcriptome sequencing, development of genome wide sequence based markers resources for improvement of non-model crops that are less costly than phenotyping.
非模式植物，即具有长生命周期、难以在实验室中生长或繁殖力低等一个或所有特征的物种，由于桑格测序的运行成本高昂，在早期的测序项目中就被排除在外。因此，关于它们的基因组学和关键生物学过程的信息并不充分。然而，近年来快速且经济高效的新一代测序（NGS）平台的出现，使得挖掘这些物种特有的某些特征基因结构成为可能。它还有助于深入了解基因表达和次生代谢过程的潜在机制，甚至在没有基因组序列信息的情况下，也促进了用于多样性表征、进化分析和标记辅助育种的基因组资源的开发。在这篇综述中，我们探讨了不同的新一代测序平台以及基于NGS的高通量基因分型技术的最新进展如何为从头全基因组/转录组测序、开发基于全基因组序列的标记资源以改良非模式作物带来回报，这些资源比表型分析成本更低。
