Han Yong-Woon, Sugiyama Hiroshi, Harada Yoshie
Institute for Integrated Cell-Materials Science (WPI-iCeMS), Kyoto University, Yoshida Honmachi, Sakyo, Kyoto 606-8501, Japan.
Biomater Sci. 2016 Mar;4(3):391-9. doi: 10.1039/c5bm00214a.
N-Methylpyrrole (Py)-N-methylimidazole-(Im) polyamides are sequence-specific DNA-binding modules that are widely used for gene regulation, as synthetic transcriptional factors and as sequence-specific DNA alkylating agents. Recently, Py-Im polyamides have been conjugated with fluorophores, resulting in conjugates that are useful for the detection of specific DNA sequences. A Förster resonance energy transfer has been observed between Cy3- and Cy5-conjugated Py-Im polyamides on the nucleosome, indicating that fluorescence-conjugated Py-Im polyamides could possibly be used to characterise protein-DNA complexes. In this minireview, we discuss recent reports regarding fluorescence-conjugated Py-Im polyamides and their future application in the characterization of protein-DNA complex formation.
N-甲基吡咯（Py）-N-甲基咪唑（Im）聚酰胺是序列特异性DNA结合模块，作为合成转录因子和序列特异性DNA烷基化剂被广泛用于基因调控。最近，Py-Im聚酰胺已与荧光团共轭，产生了可用于检测特定DNA序列的共轭物。在核小体上观察到Cy3和Cy5共轭的Py-Im聚酰胺之间存在Förster共振能量转移，这表明荧光共轭的Py-Im聚酰胺可能用于表征蛋白质-DNA复合物。在本综述中，我们讨论了关于荧光共轭Py-Im聚酰胺的最新报道及其在蛋白质-DNA复合物形成表征中的未来应用。
