Eisenmann D M, Dollard C, Winston F
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Cell. 1989 Sep 22;58(6):1183-91. doi: 10.1016/0092-8674(89)90516-3.
Mutations in the S. cerevisiae SPT15 gene were isolated as suppressors of insertion mutations that alter the transcription of adjacent genes. Molecular and genetic analysis of the cloned SPT15 gene has shown that it is the same as the gene that encodes the TATA binding factor TFIID. Analysis of spt15 mutants has demonstrated that alterations in TFIID can change transcription initiation in vivo. In addition, we demonstrate that TFIID is essential for growth and that spt15 mutations are pleiotropic, as spt15 mutants grow slowly and have defects in both mating and sporulation. Therefore, TFIID is an essential transcription factor in vivo, likely to be required for normal expression of a large number of genes.
酿酒酵母SPT15基因中的突变作为插入突变的抑制子被分离出来，这些插入突变会改变相邻基因的转录。对克隆的SPT15基因进行的分子和遗传分析表明，它与编码TATA结合因子TFIID的基因相同。对spt15突变体的分析表明，TFIID的改变可在体内改变转录起始。此外，我们证明TFIID对生长至关重要，并且spt15突变具有多效性，因为spt15突变体生长缓慢，在交配和孢子形成方面都存在缺陷。因此，TFIID是体内一种必需的转录因子，可能是大量基因正常表达所必需的。
