Groves Mitchell D, Crouch Bethany, Coombs Geoffrey W, Jordan David, Pang Stanley, Barton Mary D, Giffard Phil, Abraham Sam, Trott Darren J
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Australia.
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, Australia.
PLoS One. 2016 Jan 6;11(1):e0146034. doi: 10.1371/journal.pone.0146034. eCollection 2016.
This work investigated the molecular epidemiology and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) isolated from veterinarians in Australia in 2009. The collection (n = 44) was subjected to extensive molecular typing (MLST, spa, SCCmec, dru, PFGE, virulence and antimicrobial resistance genotyping) and antimicrobial resistance phenotyping by disk diffusion. MRSA was isolated from Australian veterinarians representing various occupational emphases. The isolate collection was dominated by MRSA strains belonging to clonal complex (CC) 8 and multilocus sequence type (ST) 22. CC8 MRSA (ST8-IV [2B], spa t064; and ST612-IV [2B], spa variable,) were strongly associated with equine practice veterinarians (OR = 17.5, 95% CI = 3.3-92.5, P < 0.001) and were often resistant to gentamicin and rifampicin. ST22-IV [2B], spa variable, were strongly associated with companion animal practice veterinarians (OR = 52.5, 95% CI = 5.2-532.7, P < 0.001) and were resistant to ciprofloxacin. A single pig practice veterinarian carried ST398-V [5C2], spa t1451. Equine practice and companion animal practice veterinarians frequently carried multiresistant-CC8 and ST22 MRSA, respectively, whereas only a single swine specialist carried MRSA ST398. The presence of these strains in veterinarians may be associated with specific antimicrobial administration practices in each animal species.
这项研究调查了2009年从澳大利亚兽医身上分离出的耐甲氧西林金黄色葡萄球菌（MRSA）的分子流行病学和抗菌药物耐药性。收集了44份样本，对其进行了广泛的分子分型（多位点序列分型、spa分型、葡萄球菌染色体盒式甲氧西林酶基因分型、dru分型、脉冲场凝胶电泳、毒力和抗菌药物耐药性基因分型），并通过纸片扩散法进行了抗菌药物耐药性表型分析。MRSA分离自代表不同职业重点的澳大利亚兽医。分离株主要为属于克隆复合体（CC）8和多位点序列类型（ST）22的MRSA菌株。CC8 MRSA（ST8-IV [2B]，spa t064；以及ST612-IV [2B]，spa可变型）与马科动物执业兽医密切相关（比值比=17.5，95%置信区间=3.3-92.5，P<0.001），且通常对庆大霉素和利福平耐药。ST22-IV [2B]，spa可变型，与伴侣动物执业兽医密切相关（比值比=52.5，95%置信区间=5.2-532.7，P<0.001），并对环丙沙星耐药。一名猪科动物执业兽医携带ST398-V [5C2]，spa t1451。马科动物执业兽医和伴侣动物执业兽医分别经常携带多重耐药的CC8和ST22 MRSA，而只有一名猪科动物专家携带MRSA ST398。兽医体内这些菌株的存在可能与每种动物物种特定的抗菌药物给药方式有关。
