Goto M, Zeller W P, Mathews H, Hurley R M
Department of Pediatrics, Loyola University of Chicago Stritch School of Medicine, Maywood, Illinois 60315.
Circ Shock. 1989 Aug;28(4):357-67.
The incidence and mortality of gram negative sepsis/septic shock remains high in newborns. In this paper, we postulated that prophylactic immunotherapy may be beneficial in newborn endotoxicosis. Lipopolysaccharide (LPS) tolerant rats were produced by serial Salmonella enteritidis LPS injections, and their sera were used for immunotherapy. Antiserum cotreatment blunted hypoglycemia and lactacidemia in S. enteritidis endotoxicosis and decreased the mortality. In 0-day-old rats, antiserum cotreatment groups had a decreased mortality when injected with S. enteritidis LPS (P less than .001), S. typhosa LPS (P less than .005), and Escherichia coli LPS (P less than .05). This global protection suggested that antibody to S. enteritidis LPS could be due to a common LPS antigen. Prophylactic antiserum administration to pregnant rats on the 18th day of gestation decreased the mortality of S. enteritidis endotoxicosis in 0-day-old rats (P less than .001). The prophylactic effects of antiserum was achieved by transplacental passage to the conceptus. We conclude that prophylactic as well simultaneous immunotherapy was beneficial in newborn rat endotoxicosis.
革兰氏阴性菌败血症/脓毒性休克在新生儿中的发病率和死亡率仍然很高。在本文中，我们推测预防性免疫疗法可能对新生儿内毒素血症有益。通过连续注射肠炎沙门氏菌脂多糖（LPS）制备LPS耐受大鼠，并将其血清用于免疫治疗。抗血清联合治疗减轻了肠炎沙门氏菌内毒素血症中的低血糖和乳酸血症，并降低了死亡率。在0日龄大鼠中，抗血清联合治疗组在注射肠炎沙门氏菌LPS（P<0.001）、伤寒沙门氏菌LPS（P<0.005）和大肠杆菌LPS（P<0.05）时死亡率降低。这种全面的保护表明，针对肠炎沙门氏菌LPS的抗体可能归因于一种共同的LPS抗原。在妊娠第18天给孕鼠预防性注射抗血清可降低0日龄大鼠肠炎沙门氏菌内毒素血症的死亡率（P<0.001）。抗血清的预防作用是通过经胎盘传递给胎儿实现的。我们得出结论，预防性以及同时进行的免疫疗法对新生大鼠内毒素血症有益。
