Barrias Cristina C
Annu Int Conf IEEE Eng Med Biol Soc. 2015 Aug;2015:1737-40. doi: 10.1109/EMBC.2015.7318713.
The delivery of cells, namely of stem cells, from biomaterial-based vehicles has been used to promote and accelerate functional tissue regeneration. Carrier materials can transport and localize cells into target sites, increase their survival and engraftment, and provide a supportive matrix to assist cellular assembly into a newly formed tissue. Hydrogels are amongst the most widely used carrier materials, as they can entrap and protect cells within a real three-dimensional (3D) microenvironment, and intrinsically present many structural properties of the natural extracellular matrix (ECM). To recreate important features of the native ECM, hydrogels may be bio-functionalized with specific cell-instructive moieties for improved biomimetic properties. The biophysical properties of hydrogels can also be tuned, increasing their versatility, as it is currently recognized that stem cells respond to their biomechanical environment. Here, an overview of some strategies commonly employed in the preparation of biomimetic matrices is presented, using alginate as a model hydrogel.
从基于生物材料的载体中递送细胞，即干细胞，已被用于促进和加速功能性组织再生。载体材料可以将细胞运输并定位到目标部位，提高其存活率和植入率，并提供支持性基质以协助细胞组装成新形成的组织。水凝胶是使用最广泛的载体材料之一，因为它们可以在真实的三维（3D）微环境中捕获和保护细胞，并且本质上具有天然细胞外基质（ECM）的许多结构特性。为了重现天然ECM的重要特征，水凝胶可以用特定的细胞指导部分进行生物功能化，以改善其仿生特性。水凝胶的生物物理特性也可以进行调整，增加其多功能性，因为目前人们认识到干细胞会对其生物力学环境做出反应。在此，以藻酸盐为模型水凝胶，概述了仿生基质制备中常用的一些策略。
