Park D J, Senok S S, Goo Y S
Annu Int Conf IEEE Eng Med Biol Soc. 2015;2015:3351-4. doi: 10.1109/EMBC.2015.7319110.
It is known that with retinal degeneration there is rewiring of retinal networks. Consequently, electrical stimulation of the degenerating retina produces responses that differ according to the stage of retinal degeneration. We sought to delineate a degeneration stage-specific parameter for the response pattern of retinal ganglion cell (RGC) spikes as a strategy for stage-specific electrical stimulation for perceptual efficiency of prosthetic vision devices. Electrically-evoked RGC spikes were recorded at different degeneration stages in the rd10 mouse model for human retinitis pigmentosa (RP). Retinal explants mounted on an 8×8 multi-electrode array were stimulated by applying 1 Hz cathodic-phase first biphasic current pulses. RGC firing rate during the first 100 ms post-stimulus was compared to that during the 100-1000 ms period and a response ratio of 100 ms (RR100 ms) was calculated through the different postnatal weeks. Our results show that during post-stimulus 100-1000 ms, the degree of correlation between pulse amplitude and evoked RGC spikes drastically decreases at PNW 4.5. This pattern was closely matched by the RR100 ms curve at this stage. We conclude that the RR100 ms might be a good indicator of the therapeutic potential of a retinal electrical prosthesis.
众所周知，随着视网膜变性，视网膜网络会发生重新布线。因此，对变性视网膜进行电刺激会产生根据视网膜变性阶段而不同的反应。我们试图为视网膜神经节细胞（RGC）尖峰的反应模式描绘一个变性阶段特异性参数，作为提高假体视觉装置感知效率的阶段特异性电刺激策略。在用于人类色素性视网膜炎（RP）的rd10小鼠模型的不同变性阶段记录电诱发的RGC尖峰。将安装在8×8多电极阵列上的视网膜外植体通过施加1Hz阴极相优先双相电流脉冲进行刺激。将刺激后前100ms内的RGC放电率与100 - 1000ms期间的放电率进行比较，并计算不同出生后周数的100ms反应率（RR100ms）。我们的结果表明，在刺激后100 - 1000ms期间，在出生后第4.5周时，脉冲幅度与诱发的RGC尖峰之间的相关程度急剧下降。此阶段RR100ms曲线与该模式紧密匹配。我们得出结论，RR100ms可能是视网膜电假体治疗潜力的良好指标。
