Higashi K, Miki N
Annu Int Conf IEEE Eng Med Biol Soc. 2015;2015:5900-3. doi: 10.1109/EMBC.2015.7319734.
This paper proposes a new method for generating microcarries from bacterial cellulose (BC). BC, which is produced by specific bacteria, is a hydrogel composed of a three dimensional network structure formed by cellulose nanofibers. BC as an ECM-like nanofibrous material exhibits an excellent environment for cellular adhesion. Moreover, BC has a high biocompatibility and mechanical strength. From these properties, BC is expected to be applied for microcarriers, which is used for cultivating anchorage-dependent cells. Then, we developed a microfabrication process to create BC microcarriers by using gelatin microspheres as sacrificial architectures. In addition, the monodispersity of the formed BC microcarreirs was investigated.
本文提出了一种由细菌纤维素（BC）制备微载体的新方法。BC是由特定细菌产生的水凝胶，由纤维素纳米纤维形成的三维网络结构组成。作为一种类似细胞外基质的纳米纤维材料，BC展现出了优异的细胞黏附环境。此外，BC具有高生物相容性和机械强度。基于这些特性，BC有望应用于用于培养贴壁依赖性细胞的微载体。然后，我们开发了一种微制造工艺，以明胶微球作为牺牲结构来制备BC微载体。此外，还研究了所形成的BC微载体的单分散性。
