[激光辅助选择活力尚可但不动的精子用于卵胞浆内单精子注射后的成功妊娠：2例报告]
[Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: A report of 2 cases].
作者信息
Chen Huan-hua, Feng Gui-xue, Zhang Bo, Shu Jin-hui, Gan Xian-you, Zhou Hong, Lin Ruo-yun
出版信息
Zhonghua Nan Ke Xue. 2015 Nov;21(11):988-91.
OBJECTIVE
To investigate the feasibility and clinical application value of selecting viable spermatozoa by noncontact diode laser.
METHODS
We obtained immotile spermatozoa from 2 infertile men with obstructive azoospermia or severe asthenospermia and selected viable spermatozoa using a single laser shot at the sperm tail. Those that responded to the laser shot by a curling reaction of the tail were regarded as presumably viable and used for intracytoplasmic sperm injection (ICSI).
RESULTS
The mean fertilization rate was 88.89% after ICSI with the laser-selected viable spermatozoa. Both of the embryo transfers resulted in a single pregnancy.
CONCLUSION
Noncontact diode laser is a useful alternative for the assessment of sperm viability, which may help to achieve successful pregnancy.
目的
探讨非接触式二极管激光筛选活精子的可行性及临床应用价值。
方法
从2例梗阻性无精子症或严重弱精子症的不育男性中获取不活动精子，通过对精子尾部单次激光照射筛选活精子。尾部出现卷曲反应的精子被视为可能存活，并用于卵胞浆内单精子注射（ICSI）。
结果
使用激光筛选的活精子进行ICSI后，平均受精率为88.89%。两次胚胎移植均导致单胎妊娠。
结论
非接触式二极管激光是评估精子活力的一种有用的替代方法，可能有助于实现成功妊娠。
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