Bungartz Annemarie, Klaus Marius, Mathew Boby, Léon Jens, Naz Ali Ahmad
University of Bonn, Institute of Crop Science and Resource Conservation, Crop Genetics and Biotechnology Unit, Katzenburgweg 5, 53115 Bonn, Germany.
University of Bonn, Institute of Crop Science and Resource Conservation, Crop Genetics and Biotechnology Unit, Katzenburgweg 5, 53115 Bonn, Germany.
Genomics. 2016 Mar;107(2-3):100-107. doi: 10.1016/j.ygeno.2015.12.007. Epub 2015 Dec 29.
The aim of the present study was to develop a new cost effective PCR based CAPS marker set using advantages of high-throughput SNP genotyping. Initially, SNP survey was made using 20 diverse barley genotypes via 9k iSelect array genotyping that resulted in 6334 polymorphic SNP markers. Principle component analysis using this marker data showed fine differentiation of barley diverse gene pool. Till this end, we developed 200 SNP derived CAPS markers distributed across the genome covering around 991cM with an average marker density of 5.09cM. Further, we genotyped 68 CAPS markers in an F2 population (Cheri×ICB181160) segregating for seed color variation in barley. Genetic mapping of seed color revealed putative linkage of single nuclear gene on chromosome 1H. These findings showed the proof of concept for the development and utility of a newer cost effective genomic tool kit to analyze broader genetic resources of barley worldwide.
本研究的目的是利用高通量SNP基因分型的优势，开发一套新的基于PCR的成本效益高的CAPS标记。最初，通过9k iSelect阵列基因分型对20种不同的大麦基因型进行SNP检测，结果得到6334个多态性SNP标记。使用这些标记数据进行主成分分析，显示了大麦不同基因库的良好分化。到目前为止，我们开发了200个源自SNP的CAPS标记，分布在整个基因组中，覆盖约991cM，平均标记密度为5.09cM。此外，我们在一个F2群体（Cheri×ICB181160）中对68个CAPS标记进行基因分型，该群体在大麦种子颜色变异方面存在分离。种子颜色的遗传图谱显示1H染色体上有一个单基因的推定连锁。这些发现证明了开发和使用一种更新的成本效益高的基因组工具包来分析全球大麦更广泛遗传资源的概念。
