Timoshenko A V, Lanteigne J, Kozak K
Department of Biology, Western University, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Mol Cell Biochem. 2016 Feb;413(1-2):137-43. doi: 10.1007/s11010-015-2647-0. Epub 2016 Jan 6.
Galectins, a family of soluble β-galactoside-binding proteins, are involved in the regulation of various cellular functions, which are essential for adaptive cellular stress responses (CSRs). Although expression patterns of galectins and galectin-binding glycans change during tissue development and cancer, the requirement and role of galectin networks in the CSRs are not completely understood. In this study, we report that the treatment of human promyelocytic HL-60 cells with stimuli mimicking hypoxia (CoCl2), inducing the endoplasmic reticulum stress (tunicamycin), and stimulating cell differentiation, result in stress-specific differential expression of galectin transcripts. In addition, we show that CoCl2 increases the expression of cell surface glycans recognized by both β-galactoside- and GlcNAc-binding lectins. Thus, microenvironmental stress changes the glycobiological status of cells representing expression profiles of endogenous lectins and corresponding glycans. These findings introduce a novel classification of galectins in HL-60 cells, which suggests diverse functions of galectin members in CSRs.
半乳糖凝集素是一类可溶性β-半乳糖苷结合蛋白，参与多种细胞功能的调节，而这些功能对于适应性细胞应激反应（CSR）至关重要。尽管在组织发育和癌症过程中半乳糖凝集素及其结合聚糖的表达模式会发生变化，但半乳糖凝集素网络在CSR中的需求和作用尚未完全明确。在本研究中，我们报道用模拟缺氧（氯化钴）、诱导内质网应激（衣霉素）以及刺激细胞分化的刺激物处理人早幼粒白血病HL-60细胞，会导致半乳糖凝集素转录本出现应激特异性差异表达。此外，我们表明氯化钴增加了同时被β-半乳糖苷结合凝集素和GlcNAc结合凝集素识别的细胞表面聚糖的表达。因此，微环境应激改变了代表内源性凝集素和相应聚糖表达谱的细胞糖生物学状态。这些发现引入了HL-60细胞中半乳糖凝集素的一种新分类，这表明半乳糖凝集素成员在CSR中具有多种功能。
