Magee D M, Wing E J
Department of Medicine, University of Texas Health Science Center, San Antonio 78284.
J Immunol. 1989 Oct 1;143(7):2336-41.
CSF have been postulated to be important mediators of host defenses. The current studies were undertaken to investigate the production of CSF by Listeria-specific, T cell clones and to assess the participation of CSF in anti-listerial host resistance. Listeria-specific L3T4+, Lyt-2- T cell clones were isolated and expanded by standard techniques. The clones themselves protected mice from listerial challenge when injected intravenously, and supernatants generated from Ag-stimulated clones were protective. In order to define factors important in the protection, supernatants from the clones were assayed for CSF by several in vitro assays. Total colony-stimulating activity was measured with a bone marrow colony-forming assay. T cell clones secreted 1000 to 2000 U/ml of colony-stimulating activity after 48 hours of stimulation with specific antigen. The relative amounts of the various CSF were determined by the capacity of supernatants to support proliferation of the factor-dependent cell lines FDCP-1 and 32D cl 3 in the presence and absence of specific anti-CSF antibodies. Results showed that most of the CSF activity was due to granulocyte-macrophage (GM)-CSF and IL-3. The role of GM-CSF in anti-listerial host resistance was assessed in two types of experiments. In one set of experiments GM-CSF activity was neutralized in the supernatants by addition of specific rabbit anti-GM-CSF antibodies. Treated and untreated supernatants were then tested for their capacity to protect nonimmune mice against listerial challenge. Neutralization of GM-CSF in the supernatants decreased the protective capacity of the supernatants by approximately 23%. In a second set of studies, the administration of recombinant murine GM-CSF was shown to protect mice from challenges of L. monocytogenes. Taken together, these experiments provide evidence that CSF are important mediators of immune T cell mediated host defenses.
脑脊液被认为是宿主防御的重要介质。目前的研究旨在调查李斯特菌特异性T细胞克隆产生脑脊液的情况，并评估脑脊液在抗李斯特菌宿主抵抗力中的作用。通过标准技术分离并扩增了李斯特菌特异性的L3T4 +、Lyt-2 - T细胞克隆。当静脉注射这些克隆时，它们本身能保护小鼠免受李斯特菌攻击，并且抗原刺激克隆产生的上清液也具有保护作用。为了确定在保护中起重要作用的因素，通过几种体外试验检测克隆上清液中的脑脊液。用骨髓集落形成试验测量总集落刺激活性。用特异性抗原刺激48小时后，T细胞克隆分泌1000至2000 U/ml的集落刺激活性。通过上清液在存在和不存在特异性抗脑脊液抗体的情况下支持因子依赖性细胞系FDCP-１和32D cl 3增殖的能力来确定各种脑脊液的相对含量。结果表明，大部分脑脊液活性归因于粒细胞 - 巨噬细胞（GM）-CSF和IL-3。在两类实验中评估了GM-CSF在抗李斯特菌宿主抵抗力中的作用。在一组实验中，通过添加特异性兔抗GM-CSF抗体来中和上清液中的GM-CSF活性。然后测试处理过和未处理过的上清液保护非免疫小鼠免受李斯特菌攻击的能力。上清液中GM-CSF的中和使上清液的保护能力降低了约23％。在第二组研究中，显示给予重组鼠GM-CSF可保护小鼠免受单核细胞增生李斯特菌的攻击。综上所述，这些实验提供了证据表明脑脊液是免疫T细胞介导的宿主防御的重要介质。
