Magee D M, Wing E J
Department of Medicine, University of Pittsburgh, School of Medicine, PA 15213.
J Immunol. 1988 Nov 1;141(9):3203-7.
Murine T lymphocyte clones sensitized to Listeria monocytogenes were developed to investigate specific mechanisms of T cell-mediated immunity. The clones were of the Thy-1.2+, L3T4+, Lyt-2- phenotype and proliferated in a dose response fashion to heat-killed Listeria. Cloned T lymphocytes injected intravenously protected nonimmune mice against L. monocytogenes challenge as determined by spleen and liver bacterial numbers. Supernatants, produced by stimulating clones with heat-killed Listeria for 48 h, also afforded protection against L. monocytogenes. The clonal supernatants contained significant quantities of IFN-gamma and CSF. IFN-gamma production was Ag specific and occurred within 24 h of stimulation. CSF production by clones was increased four- to sixfold over baseline as determined by a bone marrow colony-forming assay and was Ag specific. When the IFN-gamma in supernatants was neutralized with a specific mAb, protection afforded by the supernatants was lost. These data indicate that one mechanism for Ag-specific, T lymphocyte-mediated protection against L. monocytogenes is the release of IFN-gamma.
为了研究T细胞介导的免疫的特定机制，制备了对单核细胞增生李斯特菌敏感的小鼠T淋巴细胞克隆。这些克隆具有Thy-1.2+、L3T4+、Lyt-2-表型，并以剂量反应方式对热灭活的李斯特菌进行增殖。通过脾脏和肝脏细菌数量测定，静脉注射克隆的T淋巴细胞可保护未免疫的小鼠免受单核细胞增生李斯特菌的攻击。用热灭活的李斯特菌刺激克隆48小时产生的上清液也能提供针对单核细胞增生李斯特菌的保护。克隆上清液含有大量的IFN-γ和CSF。IFN-γ的产生具有抗原特异性，且在刺激后24小时内发生。通过骨髓集落形成试验测定，克隆产生的CSF比基线水平增加了四到六倍，且具有抗原特异性。当用特异性单克隆抗体中和上清液中的IFN-γ时，上清液提供的保护作用丧失。这些数据表明，T淋巴细胞介导的针对单核细胞增生李斯特菌的抗原特异性保护的一种机制是IFN-γ的释放。
