Ballouhey Quentin, Panicker Jalesh N, Mazerolles Catherine, Roumiguie Mathieu, Zaidi Falek, Rischmann Pascal, Malavaud Bernard, Game Xavier
Departement d'Urologie, CHU Rangueil, Toulouse, France.
Department of Uro-Neurology, UCL Institute of Neurology, The National Hospital for Neurology and Neurosurgery, Queen Square, London, WC1N 3BG, United Kingdom.
Int Braz J Urol. 2015 Nov-Dec;41(6):1141-7. doi: 10.1590/S1677-5538.IBJU.2014.0676.
To evaluate the expression of sphingosine kinase 1 (SPK1) in the bladder wall in patients with neurogenic lower urinary tract dysfunction and its association with clinical, urodynamic and pathological features.
The expression of SPK1 was studied in bladder wall specimens obtained from cystectomy using immunohistochemistry in ten patients with spinal cord injury (n=8) or multiple sclerosis (n=2) with urodynamically proven neuropathic bladder dysfunction, and in controls (n=5). Inflammation and fibrosis were analysed with histological criteria and SPK1 expression was determined by individual immunohistochemical staining.
Significant increased SPK1 urothelial immunoreactivity was shown in patients compared to control group (p=0.03). By contrast, SPK1 immunoreactivity in patients was significantly decreased in the sub-urothelium, muscles and nerves, p=0.02; 0.01 and 0.003, respectively. Patients with neurogenic detrusor overactivity (NDO) had higher SPK1 urothelium expression than those without any DO (p=0.04).
SPK1 is expressed in the human bladder wall, specifically the urothelium, in bladder specimens from patients with NDO. The role of SPK1 in the pathophysiology of NDO needs further elucidation.
评估神经源性下尿路功能障碍患者膀胱壁中鞘氨醇激酶1（SPK1）的表达及其与临床、尿动力学和病理特征的关联。
采用免疫组织化学方法，对10例经尿动力学证实为神经源性膀胱功能障碍的脊髓损伤患者（n = 8）或多发性硬化患者（n = 2）以及对照组（n = 5）膀胱切除术获取的膀胱壁标本中SPK1的表达进行研究。采用组织学标准分析炎症和纤维化情况，并通过个体免疫组织化学染色测定SPK1表达。
与对照组相比，患者的SPK1尿路上皮免疫反应性显著增加（p = 0.03）。相比之下，患者尿路上皮下层、肌肉和神经中的SPK1免疫反应性显著降低，p值分别为0.02、0.01和0.003。神经源性逼尿肌过度活动（NDO）患者的SPK1尿路上皮表达高于无任何逼尿肌过度活动的患者（p = 0.04）。
在NDO患者的膀胱标本中，SPK1在人膀胱壁中表达，特别是在尿路上皮中。SPK1在NDO病理生理学中的作用需要进一步阐明。
