Lucena Roberto G, Lima Salvador V C, Aguiar Jose L de A, Andrade Rogerson T, Pinto Flavia C M, Vilar Fabio O
Nucleo de Cirurgia Experimental do Departamento de Cirurgia da Universidade Federal de Pernambuco, Recife, Pernambuco, Brasil.
Int Braz J Urol. 2015 Nov-Dec;41(6):1148-53. doi: 10.1590/S1677-5538.IBJU.2014.0155.
To analyze the interaction between the cellulose exopolysaccharide (CEC) and urethral tissue when used as a pubovaginal sling.
Forty Wistar rats were divided into four groups. In groups A and B the cellulose exopolysaccharide (CEC) was implanted around the urethral tissue (bladder neck below the upper margin) and the rats were sacrificed at 30 and 90 days. Similar procedure was used in groups C and D using a polypropylene mesh. After sacrifice bladder and urethra were sent for histological analysis. The histological parameters (inflammatory reaction) by evaluated by quantitative analysis. For collagen deposition analysis it was used stereological method.
The cellulose exopolysaccharide (CEC) was inert and well preserved at the implanted region at the time of examination. Morphologic alterations were not found at the CEC implant but some reactions of foreign body type were observed at the adjacent structures. In some areas a process of neovascular formation was observed. Stereological analysis at the suburethral area showed a significant difference in collagen presence in favor of CEC.
The CEC implant showed adequate results when used as a suburethral sling with good integration to the host tissue, preserving its architecture.
分析用作耻骨后阴道吊带时纤维素胞外多糖（CEC）与尿道组织之间的相互作用。
将40只Wistar大鼠分为四组。A组和B组在尿道组织（上缘下方的膀胱颈）周围植入纤维素胞外多糖（CEC），并在30天和90天时处死大鼠。C组和D组采用类似方法，使用聚丙烯网片。处死大鼠后，将膀胱和尿道送去进行组织学分析。通过定量分析评估组织学参数（炎症反应）。采用体视学方法进行胶原沉积分析。
在检查时，纤维素胞外多糖（CEC）在植入区域呈惰性且保存良好。在CEC植入物处未发现形态学改变，但在相邻结构处观察到一些异物型反应。在一些区域观察到新生血管形成过程。尿道下区域的体视学分析显示，胶原存在情况有显著差异，CEC组更具优势。
当用作尿道下吊带时，CEC植入物显示出良好的效果，与宿主组织融合良好，保持了其结构。
