Moreau Marc, Néant Isabelle, Webb Sarah E, Miller Andrew L, Riou Jean-François, Leclerc Catherine
Université Toulouse 3, Centre de Biologie du Développement, 118 route de Narbonne, F31062 Toulouse Cedex 04, France; CNRS UMR5547, Toulouse F31062, France.
Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China.
Cell Calcium. 2016 Mar;59(2-3):75-83. doi: 10.1016/j.ceca.2015.12.003. Epub 2015 Dec 17.
During embryogenesis, a rise in intracellular Ca(2+) is known to be a widespread trigger for directing stem cells towards a specific tissue fate, but the precise Ca(2+) signalling mechanisms involved in achieving these pleiotropic effects are still poorly understood. In this review, we compare the Ca(2+) signalling events that appear to be one of the first steps in initiating and regulating both neural determination (neural induction) and kidney development (nephrogenesis). We have highlighted the necessary and sufficient role played by Ca(2+) influx and by Ca(2+) transients in the determination and differentiation of pools of neural or renal precursors. We have identified new Ca(2+) target genes involved in neural induction and we showed that the same Ca(2+) early target genes studied are not restricted to neural tissue but are also present in other tissues, principally in the pronephros. In this review, we also described a mechanism whereby the transcriptional control of gene expression during neurogenesis and nephrogenesis might be directly controlled by Ca(2+) signalling. This mechanism involves members of the Kcnip family such that a change in their binding properties to specific DNA sites is a result of Ca(2+) binding to EF-hand motifs. The different functions of Ca(2+) signalling during these two events illustrate the versatility of Ca(2+) as a second messenger.
在胚胎发生过程中，细胞内Ca(2+)浓度升高是引导干细胞走向特定组织命运的广泛触发因素，但实现这些多效性效应所涉及的精确Ca(2+)信号传导机制仍知之甚少。在本综述中，我们比较了Ca(2+)信号事件，这些事件似乎是启动和调节神经决定（神经诱导）和肾脏发育（肾发生）的第一步。我们强调了Ca(2+)内流和Ca(2+)瞬变在神经或肾前体细胞群的决定和分化中所起的必要和充分作用。我们确定了参与神经诱导的新的Ca(2+)靶基因，并且表明所研究的相同的Ca(2+)早期靶基因并不局限于神经组织，也存在于其他组织中，主要是在前肾中。在本综述中，我们还描述了一种机制，通过该机制神经发生和肾发生过程中基因表达的转录控制可能直接受Ca(2+)信号传导的控制。该机制涉及Kcnip家族成员，使得它们与特定DNA位点的结合特性的改变是Ca(2+)与EF手基序结合的结果。这两个事件中Ca(2+)信号传导的不同功能说明了Ca(2+)作为第二信使的多功能性。
