Waqas Muhammad, Shahzad Raheem, Khan Abdul Latif, Asaf Sajjad, Kim Yoon-Ha, Kang Sang-Mo, Bilal Saqib, Hamayun Muhammad, Lee In-Jung
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea; Department of Agriculture Extension, Buner 19290, Pakistan.
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
Plant Physiol Biochem. 2016 Feb;99:118-25. doi: 10.1016/j.plaphy.2015.12.012. Epub 2015 Dec 24.
In this study, it was hypothesized that application of triacontanol, a ubiquitous saturated primary alcohol, at different times-before (TBHS), mid (TMHS), and after (TAHS) heat stress-will extend heat stress (HS) protection in mungbean. The effect of triacontanol on the levels of defense hormones abscisic acid (ABA) and jasmonic acid (JA) was investigated along with the plant growth promotion, nutrient and amino acid content with and without heat stress. Heat stress caused a prominent reduction in plant growth attributes, nutrient and amino acid content, which were attributed to the decreased level of ABA and JA. However, application of triacontanol, particularly in the TBHS and TMHS treatments, reversed the deleterious effects of HS by showing increased ABA and JA levels that favored the significant increase in plant growth attributes, enhanced nutrient content, and high amount of amino acid. TAHS, a short-term application of triacontanol, also significantly increased ABA and JA levels and thus revealed important information of its association with hormonal modulation. The growth-promoting effect of triacontanol was also confirmed under normal growth conditions. To the best of our knowledge, this study is the first to demonstrate the beneficial effects of triacontanol, with or without heat stress, on mungbean and its interaction with or regulation of the levels of defense hormones.
在本研究中，我们假设在不同时间（热应激前（TBHS）、热应激中期（TMHS）和热应激后（TAHS））施用三十烷醇（一种普遍存在的饱和伯醇），将能延长绿豆对热应激（HS）的耐受性。我们研究了三十烷醇对防御激素脱落酸（ABA）和茉莉酸（JA）水平的影响，以及在有或无热应激情况下对植物生长促进、养分和氨基酸含量的影响。热应激导致植物生长特性、养分和氨基酸含量显著降低，这归因于ABA和JA水平的下降。然而，施用三十烷醇，特别是在TBHS和TMHS处理中，通过提高ABA和JA水平，逆转了热应激的有害影响，这有利于植物生长特性显著增加、养分含量提高以及氨基酸含量增加。TAHS（三十烷醇的短期施用）也显著提高了ABA和JA水平，从而揭示了其与激素调节关联的重要信息。三十烷醇在正常生长条件下的促生长作用也得到了证实。据我们所知，本研究首次证明了无论有无热应激，三十烷醇对绿豆的有益作用及其与防御激素水平的相互作用或调节。
