Attallah Abdelfattah M, El-Far Mohamed, Omran Mohamed M, Farid Khaled, Attallah Ahmed A, Abd-Elaziz Dalal, El-Bendary Mohamed S, El-Dosoky Ibrahim, Ismail Hisham
a R & D Department, Biotechnology Research Center , New Damietta , Egypt.
b Chemistry Department, Faculty of Science , Mansoura University , Mansoura , Egypt.
J Immunoassay Immunochem. 2016;37(3):316-30. doi: 10.1080/15321819.2015.1135163.
The goal of this study was to determine the levels of S. mansoni antigen in different liver fibrosis stages with chronic hepatitis C (CHC) Egyptian patients. A total of 174 CHC patients showing HCV-NS4 antigen and HCV- RNA in their sera were included. S. mansoni antigen was detected in serum using Western blot and ELISA. The levels of interferon-γ (IFN- γ) were determined using ELISA. The 50 kDa S. mansoni antigen discriminated patients infected with S. mansoni from healthy individuals with 0.93 area under curve (AUC), 92% sensitivity, and 97% specificity. The level of S. mansoni antigen (μg/ml) was significantly (P < 0.0001) increased with the progression of liver fibrosis stages (26.9 ± 17.5 in F1, 42.1 ± 25.2 in F2, 49.8 ± 30.3 in F3 and 62.2 ± 26.3 μg/mL in F4 liver cirrhosis), 26.9 ± 17.59 in significant fibrosis (F2-F4); 51.2 ± 27.9 in advanced fibrosis (F3-F4). A significant correlation (r = 0.506; P < 0.0001) was shown between the levels of the S. mansoni antigen and the HCV-NS4 antigen. In conclusion, the presence of S. mansoni antigen in different liver fibrosis stages of CHC patients confirming that concomitant schistosome infection aggravates liver disease.
本研究的目的是确定慢性丙型肝炎（CHC）埃及患者不同肝纤维化阶段曼氏血吸虫抗原的水平。共纳入174例血清中显示丙肝病毒非结构蛋白4（HCV-NS4）抗原和丙肝病毒核糖核酸（HCV-RNA）的CHC患者。采用蛋白质印迹法和酶联免疫吸附测定法（ELISA）检测血清中的曼氏血吸虫抗原。采用ELISA测定干扰素-γ（IFN-γ）水平。50 kDa曼氏血吸虫抗原区分曼氏血吸虫感染患者与健康个体的曲线下面积（AUC）为0.93，灵敏度为92%，特异性为97%。随着肝纤维化阶段的进展，曼氏血吸虫抗原水平（μg/ml）显著升高（F1期为26.9±17.5，F2期为42.1±25.2，F3期为49.8±30.3，F4期肝硬化为62.2±26.3 μg/mL），显著纤维化（F2-F4）时为26.9±17.59；晚期纤维化（F3-F4）时为51.2±27.9。曼氏血吸虫抗原水平与HCV-NS4抗原水平之间存在显著相关性（r = 0.506；P < 0.0001）。总之，CHC患者不同肝纤维化阶段存在曼氏血吸虫抗原，证实合并血吸虫感染会加重肝脏疾病。
