Department of Civil and Environmental Engineering, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Department of Civil and Environmental Engineering, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
J Hazard Mater. 2016 Apr 5;306:247-256. doi: 10.1016/j.jhazmat.2015.12.016. Epub 2015 Dec 17.
Crude (4-methylcyclohexyl)methanol (MCHM) caused extensive contamination of drinking water, wastewater, and the environment during the 2014 West Virginia Chemical Spill. However, information related to the environmental degradation of cis- and trans-4-MCHM, the main components of the crude 4-MCHM mixture, remains largely unknown. This study is among the first to investigate the degradation kinetics and transformation of 4-MCHM isomers in activated sludge. The 4-MCHM loss was mainly due to biodegradation to form carbon dioxide (CO2), plus acetic, propionic, isobutyric, and isovaleric acids with little contribution from adsorption. The biodegradation of 4-MCHM isomers followed the first-order kinetic model with half-lives higher than 0.50 days. Nitrate augmented the degradation of 4-MCHM isomers, while glucose and acetate decreased their degradation. One 4-MCHM-degrading bacterium isolated from activated sludge was identified as Acinetobacter bouvetii strain EU40 based on 16S rRNA gene sequences. This study will enhance the prediction of the environmental fate of 4-MCHM in water treatment systems.
粗（4-甲基环己基）甲醇（MCHM）在 2014 年西弗吉尼亚州化学泄漏事件中，导致饮用水、废水和环境受到广泛污染。然而，有关顺式和反式 4-MCHM（粗 4-MCHM 混合物的主要成分）的环境降解信息在很大程度上仍然未知。本研究首次调查了 4-MCHM 异构体在活性污泥中的降解动力学和转化。4-MCHM 的损失主要归因于生物降解形成二氧化碳（CO2），以及少量来自吸附的乙酸、丙酸、异丁酸和异戊酸。4-MCHM 异构体的生物降解遵循一级动力学模型，半衰期高于 0.50 天。硝酸盐促进了 4-MCHM 异构体的降解，而葡萄糖和乙酸则降低了它们的降解。从活性污泥中分离出的一株 4-MCHM 降解菌，根据 16S rRNA 基因序列鉴定为鲍氏不动杆菌 EU40 株。本研究将增强对水处理系统中 4-MCHM 环境归宿的预测。
