Dudek H, Reddy E P
Wistar Institute, Philadelphia, Pennsylvania 19104.
Oncogene. 1989 Sep;4(9):1061-6.
The c-myb gene is the normal cellular homolog of v-myb, the oncogenic component of Avian Myeloblastosis Virus (AMV). The c-myb gene has previously been shown to code for a single protein species of about 75 kd. However, accumulating evidence indicates that this gene could code for multiple mRNAs as a result of differential splicing. This was first detected in the ABPL-2 tumor line and was later shown to occur in normal cells. To test if indeed these differentially spliced mRNAs code for a new protein species that went hitherto undetected, a series of antibodies directed against specific domains of the myb protein, including the putative sequences that could be generated from the alternatively spliced mRNAs were generated. Immunoprecipitation analysis using these antibodies show that both normal and tumor cell lines synthesize at least two myb proteins, one of 75 kd and the other of 89 kd.
c-myb基因是禽成髓细胞瘤病毒（AMV）致癌成分v-myb的正常细胞同源物。先前已证明c-myb基因编码一种约75kd的单一蛋白质。然而，越来越多的证据表明，由于可变剪接，该基因可能编码多种mRNA。这首先在ABPL-2肿瘤细胞系中被检测到，后来证明在正常细胞中也会发生。为了测试这些可变剪接的mRNA是否确实编码一种迄今未被发现的新蛋白质，人们制备了一系列针对myb蛋白特定结构域的抗体，包括可能由可变剪接mRNA产生的假定序列。使用这些抗体进行的免疫沉淀分析表明，正常细胞系和肿瘤细胞系都至少合成两种myb蛋白，一种为75kd，另一种为89kd。
