Laget M P, Callebaut I, de Launoit Y, Stehelin D, Mornon J P
Unité d'Oncologie Moléculaire, CNRS URA 1160, Institut Pasteur, Lille, France.
Nucleic Acids Res. 1993 Dec 25;21(25):5987-96. doi: 10.1093/nar/21.25.5987.
The Ets family of transcription factors shares a 85 amino acid domain, named the ETS domain, which appears responsible for their DNA binding activity. This domain did not show any clear similarity with already known DNA binding motifs. Hydrophobic Cluster Analysis (HCA), a sensitive method able to detect protein structural relationships even at low sequence identity, was chosen in order to compare the ETS domain with other conventional DNA binding motifs. HCA analysis combined with known three-dimensional NMR data, suggests that the ETS domain may be structurally related to the Myb DNA binding domain and possibly to the HMG one. Indeed, the ETS domain is likely to contain two helix-loop-helix motifs.
转录因子的Ets家族共有一个含85个氨基酸的结构域，称为ETS结构域，该结构域似乎决定了它们的DNA结合活性。此结构域与已知的DNA结合基序没有任何明显的相似性。为了将ETS结构域与其他传统的DNA结合基序进行比较，我们选择了疏水簇分析（HCA），这是一种即使在低序列同一性时也能检测蛋白质结构关系的灵敏方法。HCA分析结合已知的三维核磁共振数据表明，ETS结构域可能在结构上与Myb DNA结合结构域相关，甚至可能与HMG结构域相关。实际上，ETS结构域可能包含两个螺旋-环-螺旋基序。
