King's College London Dental Institute, Guy's, King's and St. Thomas' Hospitals, London SE1 9RT, UK.
National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK.
Dent Mater. 2016 Feb;32(2):278-84. doi: 10.1016/j.dental.2015.11.016. Epub 2015 Dec 31.
This paper investigates the application of confocal laser scanning microscopy to determine the effect of acid-mediated erosive enamel wear on the micro-texture of polished human enamel in vitro.
Twenty polished enamel samples were prepared and subjected to a citric acid erosion and pooled human saliva remineralization model. Enamel surface microhardness was measured using a Knoop hardness tester, which confirmed that an early enamel erosion lesion was formed which was then subsequently completely remineralized. A confocal laser scanning microscope was used to capture high-resolution images of the enamel surfaces undergoing demineralization and remineralization. Area-scale analysis was used to identify the optimal feature size following which the surface texture was determined using the 3D (areal) texture parameter Sa.
The Sa successfully characterized the enamel erosion and remineralization for the polished enamel samples (P<0.001).
Areal surface texture characterization of the surface events occurring during enamel demineralization and remineralization requires optical imaging instrumentation with lateral resolution <2.5 μm, applied in combination with appropriate filtering in order to remove unwanted waviness and roughness. These techniques will facilitate the development of novel methods for measuring early enamel erosion lesions in natural enamel surfaces in vivo.
本研究旨在应用共聚焦激光扫描显微镜来确定酸介导的侵蚀性釉质磨损对体外抛光人釉质微观结构的影响。
制备 20 个抛光釉质样本，并进行柠檬酸侵蚀和人唾液再矿化模型实验。使用努普硬度计测量釉质表面显微硬度，证实形成早期釉质侵蚀病变，随后完全再矿化。使用共聚焦激光扫描显微镜捕获经历脱矿和再矿化的釉质表面的高分辨率图像。采用面积尺度分析确定最佳特征尺寸，然后使用 3D（面积）纹理参数 Sa 确定表面纹理。
Sa 成功地对抛光釉质样本的釉质侵蚀和再矿化进行了特征描述（P<0.001）。
为了对釉质脱矿和再矿化过程中发生的表面事件进行面积表面纹理特征描述，需要使用横向分辨率<2.5 μm 的光学成像仪器，并结合适当的滤波，以去除不需要的波纹和粗糙度。这些技术将有助于开发在天然釉质表面体内测量早期釉质侵蚀病变的新方法。
