Yuan Fang, Lin Long-Xiang, Zhang Hui-Hui, Huang Dan, Sun Yu-Long
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.
J Biomed Mater Res A. 2016 May;104(5):1175-81. doi: 10.1002/jbm.a.35653. Epub 2016 Feb 2.
Adhesions often occur after abdominal surgery. It could cause chronic pelvic pain, intestinal obstruction, and infertility. A hydrogel biomaterial, carbodiimide-derivatized hyaluronic acid gelatin (cd-HA gelatin), has been successfully used to reduce adhesion formation after flexor tendon grafting. This study investigated the efficacy of cd-HA gelatin in preventing postsurgical peritoneal adhesions in a rat model. The surgical traumas were created on the underlying muscle of the abdominal wall and the serosal layer of the cecum. The wounds were covered with or without cd-HA gelatin. Animals were euthanized at day 14 after surgery. Adhesion formation was assessed with adhesion degree and adhesion breaking strength. The healing of abdominal wall was evaluated with biomechanical testing and histological analysis. The adhesions occurred in all rats (n = 12) without cd-HA gelatin treatment. The application of cd-HA gelatin significantly reduced the adhesion rate from 100% to 58%. The decrease of adhesion breaking strength also manifested that cd-HA gelatin could reduce postsurgical intra-abdominal adhesion formation. Moreover, it was found that cd-HA gelatin was a safe material and could promote tissue healing. The cd-HA gelatin hydrogel could reduce the formation of intra-abdominal adhesions without adversely effects on wound healing.
粘连常在腹部手术后发生。它可能导致慢性盆腔疼痛、肠梗阻和不孕。一种水凝胶生物材料，碳二亚胺衍生化透明质酸明胶（cd-HA明胶），已成功用于减少屈肌腱移植后的粘连形成。本研究在大鼠模型中研究了cd-HA明胶预防术后腹膜粘连的效果。在腹壁下层肌肉和盲肠浆膜层制造手术创伤。伤口覆盖有或没有cd-HA明胶。术后第14天对动物实施安乐死。用粘连程度和粘连断裂强度评估粘连形成情况。通过生物力学测试和组织学分析评估腹壁的愈合情况。未用cd-HA明胶治疗的所有大鼠（n = 12）均发生了粘连。cd-HA明胶的应用显著降低了粘连率，从100%降至58%。粘连断裂强度的降低也表明cd-HA明胶可减少术后腹腔内粘连形成。此外，发现cd-HA明胶是一种安全的材料，可促进组织愈合。cd-HA明胶水凝胶可减少腹腔内粘连的形成，且对伤口愈合无不利影响。
