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有机酸、去乙酰车叶草苷酸和多酚类化合物分析作为诺丽果(海巴戟)产品特性鉴定的潜在工具

Analysis of Organic Acids, Deacetyl Asperulosidic Acid and Polyphenolic Compounds as a Potential Tool for Characterization of Noni (Morinda citrifolia) Products.

作者信息

Bittová Miroslava, Hladůkova Dita, Roblová Vendula, Krácmar Stanislav, Kubán Petr, Kubán Vlastimil

出版信息

Nat Prod Commun. 2015 Nov;10(11):1817-20.

Abstract

Organic acids, deacetyl asperulosidic acid (DAA) and polyphenolic compounds in various noni (Morinda citrifolia L.) products (4 juices, 4 dry fruit powders and 2 capsules with dry fruit powder) were analyzed. Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a variable wavelength detector (VWD) and electrospray ionization time-of-flight mass spectrometer (ESI-TOF MS) was applied for simultaneous analysis of organic acids (malic, lactic, citric and succinic acid) and DAA. An RP-HPLC method with diode-array detector (DAD) was developed for the analysis of polyphenolic compound content (rutin, catechin, quercitrin, kaempferol, gallic acid, caffeic acid and p-coumaric acid). The developed methods can contribute to better characterization of available noni products that is required from the consumers. In our study, we discovered significant dissimilarities in the content of DAA, citric acid and several phenolic compounds in some samples.

摘要

对各种诺丽(海巴戟天)产品(4种果汁、4种干果粉末和2种含干果粉末的胶囊)中的有机酸、去乙酰车叶草苷酸(DAA)和多酚类化合物进行了分析。采用反相高效液相色谱(RP-HPLC)结合可变波长检测器(VWD)和电喷雾电离飞行时间质谱仪(ESI-TOF MS)同时分析有机酸(苹果酸、乳酸、柠檬酸和琥珀酸)和DAA。建立了一种采用二极管阵列检测器(DAD)的RP-HPLC方法用于分析多酚类化合物含量(芦丁、儿茶素、槲皮苷、山奈酚、没食子酸、咖啡酸和对香豆酸)。所建立的方法有助于更好地表征消费者所要求的现有诺丽产品。在我们的研究中,我们发现一些样品中DAA、柠檬酸和几种酚类化合物的含量存在显著差异。

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