Chen Chunyuan, Li Qing, Wang Yang
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2015 Sep;29(9):1150-4.
To review the current progresses in purification strategies, biological characters, and functions of endothelial progenitor cells (EPCs) derived extracellular vesicles (EVs) (EPC-EVs).
Recent relevant publications on the EPC-EVs were extensively reviewed, analyzed, and summarized.
EPC-EVs are usually isolated by differential centrifugation and exhibit a homogenous pattern of spheroid particles with a diameter ranging from 60 to 160 nm under transmission electron microscopy. EPC-EVs are positive for cell-surface markers of EPCs (CD31, CD34, and CD133), and negative for markers of platelets (P-selectin and CD42b) and monocytes (CD14). Recent studies have shown the effectiveness of EPC-EVs in ischemic injuries, anti-Thyl glomerulonephritis, and cardiomyocyte hypertrophy, and also shown their predictive role in cardio-cerebral-vascular diseases.
An alluring prospect exists on the EPC-EVs-related research. Further studies are required to decipher the composition of EPC-EVs and their precise role in pathophysiological processes, and to investigate the molecular mechanisms for their targeting and function.
综述内皮祖细胞（EPCs）衍生的细胞外囊泡（EVs）（EPC-EVs）的纯化策略、生物学特性及功能的当前研究进展。
广泛查阅、分析并总结近期有关EPC-EVs的相关出版物。
EPC-EVs通常通过差速离心法分离，在透射电子显微镜下呈现均一的球形颗粒形态，直径范围为60至160纳米。EPC-EVs对EPCs的细胞表面标志物（CD31、CD34和CD133）呈阳性，而对血小板标志物（P-选择素和CD42b）和单核细胞标志物（CD14）呈阴性。近期研究表明EPC-EVs在缺血性损伤、抗胸腺肾小球肾炎及心肌细胞肥大方面具有有效性，还表明它们在心血管疾病中的预测作用。
EPC-EVs相关研究存在诱人的前景。需要进一步研究来阐明EPC-EVs的组成及其在病理生理过程中的精确作用，并探究其靶向和功能的分子机制。
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